Human/Mouse Phospho-STAT1 (Y701) Antibody

Catalog # Availability Size / Price Qty
AF2894
AF2894-SP
Detection of Phospho-STAT1 in IFN-alpha -treated Daudi Human Cell Line by Flow Cytometry.
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Product Details
Citations (5)
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Human/Mouse Phospho-STAT1 (Y701) Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human STAT1 when phosphorylated at Y701 in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human STAT1 Y701 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection of Phospho-STAT1 in IFN-alpha -treated Daudi Human Cell Line by Flow Cytometry. View Larger

Detection of Phospho-STAT1 in IFN-alpha -treated Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was unstimulated (light orange filled histogram) or treated with 500 units/mL Recombinant Human IFN‑ alpha (Catalog # 11100-1) for 20 minutes (dark orange filled histogram), then stained with Rabbit Anti-Human/Mouse Phospho‑STAT1 (Y701) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2894) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Western Blot Detection of Human Phospho-STAT1 (Y701) by Western Blot. View Larger

Detection of Human Phospho-STAT1 (Y701) by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN-alpha 2a for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT1 (Y701) at approximately 94 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western Detection of Human Phospho-STAT1 (Y701) by Simple WesternTM. View Larger

Detection of Human Phospho-STAT1 (Y701) by Simple WesternTM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN‑ alpha A (Catalog # 11100-1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-STAT1 (Y701) at approximately 86 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT1

STAT1 (signal transducer and activator of transcription #1) is an 88 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT1 is associated with type I and II interferon signaling. All STATs contain an N-terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT1 is phosphorylated at Y701 by receptor-associated Janus kinases (JAKs) leading to STAT1 dimerization and subsequent translocation to the nucleus to activate gene transcription.

Long Name
Signal Transducer and Activator of Transcription 1
Entrez Gene IDs
6772 (Human); 20846 (Mouse)
Alternate Names
CANDF7; DKFZp686B04100; ISGF-3; ISGF-3,91kD; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription-1; STAT1; STAT91

Product Datasheets

Citations for Human/Mouse Phospho-STAT1 (Y701) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Molecular determinants of plaque size as an indicator of dengue virus attenuation
    Sci Rep, 2016;6(0):26100.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: IHC - Not specified
  2. PD-L1 blockade enhances response of pancreatic ductal adenocarcinoma to radiotherapy
    EMBO Mol Med, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  3. Thymic DCs derived IL-27 regulates the final maturation of CD4(+) SP thymocytes
    Sci Rep, 2016;6(0):30448.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  4. Annexin V incorporated into influenza virus particles inhibits gamma interferon signaling and promotes viral replication.
    Authors: Berri F, Haffar G, Le V, Sadewasser A, Paki K, Lina B, Wolff T, Riteau B
    J Virol, 2014;88(19):11215-28.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  5. The RAS/Raf1/MEK/ERK signaling pathway facilitates VSV-mediated oncolysis: implication for the defective interferon response in cancer cells.
    Authors: Noser JA, Mael AA, Sakuma R, Ohmine S, Marcato P, Lee PW, Ikeda Y
    Mol. Ther., 2007;15(8):1531-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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