Human/Mouse Phospho-STAT1 (Y701) Antibody

R&D Systems | Catalog # AF2894

R&D Systems
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Hamster

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, Simple Western, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry, Capillary Immunoblot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all STAT1 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human STAT1 Y701 site

Specificity

Detects human STAT1 when phosphorylated at Y701 in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse Phospho-STAT1 (Y701) Antibody

Detection of Human Phospho-STAT1 (Y701) antibody by Western Blot.

Detection of Human Phospho-STAT1 (Y701) by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN-a2a for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT1 (Y701) at approximately 94 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Phospho-STAT1 antibody in IFN-a-treated Daudi Human Cell Line antibody by Flow Cytometry.

Detection of Phospho-STAT1 in IFN-alpha -treated Daudi Human Cell Line by Flow Cytometry.

Daudi human Burkitt's lymphoma cell line was unstimulated (light orange filled histogram) or treated with 500 units/mL Recombinant Human IFN-a (Catalog # 11100-1) for 20 minutes (dark orange filled histogram), then stained with Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
Detection of Human Phospho-STAT1 (Y701) antibody by Simple WesternTM.

Detection of Human Phospho-STAT1 (Y701) by Simple WesternTM.

Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN-aA (Catalog # 11100-1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-STAT1 (Y701) at approximately 86 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Mouse STAT1 by Western Blot

Detection of Mouse STAT1 by Western Blot

Effects of kurarinone on the phosphorylation of p-STAT1 and p-STAT3 in lymph nodes. Single cell suspensions were collected from ILNs on day 42, the protein expression levels of p-STAT1, STAT1, p-STAT3, and STAT3 were measured using Western blots. (A) Representative images of Western blot and (B) Densitometric analysis for protein expressions was performed using ImageJ software. Data are presented as mean ± SEM of 6 mice from one of three experiments. (*) p < 0.05, (**) p < 0.01, (***) p < 0.001 versus vehicle-treated CIA mice group (One Way ANOVA followed by Tukey’s multiple comparison test). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33924467), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Phospho-STAT1 (Y701) by Western Blot

Detection of Phospho-STAT1 (Y701) by Western Blot

The loss of C9ORF72 leads to JAK-STAT activation(A) RT-qPCR analysis of Sting levels in control and C9orf72−/− RAW264.7 cells untreated or treated with DMXAA for 16h. Data represent the mean ± SEM. Statistical significance was analyzed by two-way ANOVA (n = 3), ns = not significant, ∗p < 0.05, ∗∗p < 0.01.(B) Protein levels of STAT1 in spleen lysates from 6-month-old WT and C9orf72−/− mice were analyzed using western blot. Mixed male and female mice were used. Data represent the mean ± SEM. Statistical significance was analyzed by unpaired one-tail Student’s t test (n = 4), ns = not significant, ∗p < 0.05.(C) Protein levels of STAT1 and p-STAT1 in control or C9orf72−/− RAW264.7 cells untreated or treated with DMXAA for 2h were analyzed using western blot and normalized to GAPDH. Data represent the mean ± SEM. Statistical significance was analyzed by unpaired two-tail Student’s t test (n = 3), ns = not significant, ∗p < 0.05.(D) RT-qPCR analysis of Stat1 levels in control and C9orf72−/− RAW264.7 cells untreated or treated with DMXAA for 16h. Data represent the mean ± SEM. Statistical significance was analyzed by two-way ANOVA (n = 3), ns = not significant, ∗p < 0.05, ∗∗∗∗p < 0.0001.(E) IFN-beta levels in control and C9orf72−/− RAW264.7 cells untreated or treated with DMXAA for 16h were measured using ELISA. Data represent the mean ± SEM. Statistical significance was analyzed by two-way ANOVA (n = 3), ns = not significant, ∗∗p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37250330), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Phospho-STAT1 (Y701) by Western Blot

Detection of Phospho-STAT1 (Y701) by Western Blot

The loss of C9ORF72 leads to JAK-STAT activation(A) RT-qPCR analysis of Sting levels in control and C9orf72−/− RAW264.7 cells untreated or treated with DMXAA for 16h. Data represent the mean ± SEM. Statistical significance was analyzed by two-way ANOVA (n = 3), ns = not significant, ∗p < 0.05, ∗∗p < 0.01.(B) Protein levels of STAT1 in spleen lysates from 6-month-old WT and C9orf72−/− mice were analyzed using western blot. Mixed male and female mice were used. Data represent the mean ± SEM. Statistical significance was analyzed by unpaired one-tail Student’s t test (n = 4), ns = not significant, ∗p < 0.05.(C) Protein levels of STAT1 and p-STAT1 in control or C9orf72−/− RAW264.7 cells untreated or treated with DMXAA for 2h were analyzed using western blot and normalized to GAPDH. Data represent the mean ± SEM. Statistical significance was analyzed by unpaired two-tail Student’s t test (n = 3), ns = not significant, ∗p < 0.05.(D) RT-qPCR analysis of Stat1 levels in control and C9orf72−/− RAW264.7 cells untreated or treated with DMXAA for 16h. Data represent the mean ± SEM. Statistical significance was analyzed by two-way ANOVA (n = 3), ns = not significant, ∗p < 0.05, ∗∗∗∗p < 0.0001.(E) IFN-beta levels in control and C9orf72−/− RAW264.7 cells untreated or treated with DMXAA for 16h were measured using ELISA. Data represent the mean ± SEM. Statistical significance was analyzed by two-way ANOVA (n = 3), ns = not significant, ∗∗p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37250330), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse Phospho-STAT1 (Y701) Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: IFN-alpha -treated Daudi human Burkitt's lymphoma cell line fixed with paraformaldehyde and permeabilized with methanol

Simple Western

5 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line treated with Recombinant Human IFN‑ alpha A (Catalog # 11100-1)

Western Blot

0.5 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line treated with Recombinant Human IFN-alpha 2a

Reviewed Applications

Read 9 reviews rated 4.4 using AF2894 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STAT1

STAT1 (signal transducer and activator of transcription #1) is an 88 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT1 is associated with type I and II interferon signaling. All STATs contain an N-terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT1 is phosphorylated at Y701 by receptor-associated Janus kinases (JAKs) leading to STAT1 dimerization and subsequent translocation to the nucleus to activate gene transcription.

Long Name

Signal Transducer and Activator of Transcription 1

Alternate Names

CANDF7, ISGF-3, STAT91

Entrez Gene IDs

6772 (Human); 20846 (Mouse)

Gene Symbol

STAT1

Additional STAT1 Products

Product Documents for Human/Mouse Phospho-STAT1 (Y701) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Phospho-STAT1 (Y701) Antibody

For research use only

Citations for Human/Mouse Phospho-STAT1 (Y701) Antibody

Customer Reviews for Human/Mouse Phospho-STAT1 (Y701) Antibody (9)

4.4 out of 5
9 Customer Ratings
5 Stars
56%
4 Stars
33%
3 Stars
11%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 9 reviews Showing All
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  • Human/Mouse Phospho-STAT1 (Y701) Antibody
    Name: Marcos Paulo Oliveira Almeida
    Application: Western Blot
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Species: Human
    Verified Customer | Posted 10/12/2025
    Human/Mouse Phospho-STAT1 (Y701) Antibody AF2894
  • Human/Mouse Phospho-STAT1 (Y701) Antibody
    Name: Khuynh Nguyen
    Application: Western Blot
    Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line
    Species: Mouse
    Verified Customer | Posted 03/28/2023
    First antibody: 1:2000, 4 oC, overnight Second antibody: 1:5000, room temperature, 1 h
    Human/Mouse Phospho-STAT1 (Y701) Antibody AF2894
  • Human/Mouse Phospho-STAT1 (Y701) Antibody
    Name: Khuynh Nguyen
    Application: Western Blot
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 04/18/2022
    Human/Mouse Phospho-STAT1 (Y701) Antibody AF2894
  • Human/Mouse Phospho-STAT1 (Y701) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human lung macrophages and Jurkat
    Species: Human
    Verified Customer | Posted 04/04/2020
    Human/Mouse Phospho-STAT1 (Y701) Antibody AF2894
  • Human/Mouse Phospho-STAT1 (Y701) Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human/Mouse Phospho-STAT1 (Y701) Antibody AF2894
  • Human/Mouse Phospho-STAT1 (Y701) Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 02/19/2019
    Human/Mouse Phospho-STAT1 (Y701) Antibody AF2894
  • Human/Mouse Phospho-STAT1 (Y701) Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human and Mouse
    Verified Customer | Posted 11/08/2018
  • Human/Mouse Phospho-STAT1 (Y701) Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/02/2018
  • Human/Mouse Phospho-STAT1 (Y701) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: EMT6 cells and CT26
    Species: Mouse
    Verified Customer | Posted 10/17/2018
    Human/Mouse Phospho-STAT1 (Y701) Antibody AF2894

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