Human/Mouse/Rat ALDH2 Antibody

Catalog # Availability Size / Price Qty
MAB10168-100
MAB10168-SP
Human/Mouse/Rat ALDH2 Antibody in Western Blot
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Human/Mouse/Rat ALDH2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human ALDH2 in direct ELISAs. Detects human, mouse, and rat ALDH2 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 1007902
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Synthetic peptide containing human ALDH2
Ser18-Ser517
Accession # P05091
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
20 µg/mL
See below
Immunohistochemistry
0.5-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human. Mouse, and Rat ALDH2 by Western Blot. View Larger

Detection of Human. Mouse, and Rat ALDH2 by Western Blot. Western blot shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat ALDH2 Monoclonal Antibody (Catalog # MAB10168) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ALDH2 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry ALDH2  in SK-BR-3 Human Cell Line. View Larger

ALDH2 in SK-BR-3 Human Cell Line. ALDH2 was detected in immersion fixed SK‑BR‑3 human breast cancer cell line (positive staining) and MCF‑7 human breast cancer cell line (negative staining) using Mouse Anti-Human/Mouse/Rat ALDH2 Monoclonal Antibody (Catalog # MAB10168) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry ALDH2  in Human Kidney. View Larger

ALDH2 in Human Kidney. ALDH2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat ALDH2 Monoclonal Antibody (Catalog # MAB10168) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection  of Human, Mouse, and Rat ALDH2 by Simple WesternTM. View Larger

Detection of Human, Mouse, and Rat ALDH2 by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue, loaded at 0.2 mg/mL. A specific band was detected for ALDH2 at approximately 55 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat ALDH2 Monoclonal Antibody (Catalog # MAB10168). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALDH2

Aldehyde Dehydrogenase 2 (ALDH2) is a protein involved in the pathway which synthesizes acetate from ethanol.  It also functions as a protector against oxidative stress.  The ALDH2 isoform is localized to the mitochondrial matrix.  Genetic variation in ALDH2 in the East Asian population is associated with high instances of acute alcohol intoxication.

Long Name
Aldehyde Dehydrogenase 2 Family Member
Entrez Gene IDs
217 (Human); 11669 (Mouse)
Alternate Names
acetaldehyde dehydrogenase 2; aldehyde dehydrogenase 2 family (mitochondrial); aldehyde dehydrogenase, mitochondrial; ALDH class 2; ALDH2; ALDH-E2; ALDHI; ALDM; EC 1.2.1; EC 1.2.1.3; liver mitochondrial ALDH; MGC1806; nucleus-encoded mitochondrial aldehyde dehydrogenase 2

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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