The antibody detects human, mouse and rat Annexin A1 in Western blots. In Western blots, goat anti‑human/mouse/rat Annexin A1 does not cross‑react with recombinant human Annexin A2, A3, A4, A6, A7, A8, A9, A10, A11 or A13.
Polyclonal Goat IgG
E. coli-derived recombinant human Annexin A1 Met1-Asn346 Accession # P04083
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human/Mouse/Rat Annexin A1 by Western Blot.
Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Annexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3770) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Annexin A1 at approximately 35-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Annexin A1/Annexin I was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human/Mouse/Rat Annexin A1/Annexin I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3770) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human and Mouse Annexin A1 by Simple WesternTM.
Simple Western lane view shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Annexin A1 at approximately 44 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse/Rat Annexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3770) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Annexin A1
The Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexin’s have a molecular weight of approximately 35 to 40 kDa and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites. The C‑terminal domain is comprised of four 60‑70 amino acid repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats). The four annexin repeats form a highly alpha -helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner. Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. Annexin A1 (ANXA1), also known as annexin I, lipocortin I, and calpactin II, is an ~ 40 kDa protein with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, Annexin A1 may have anti-inflammatory activity. Human Annexin A1 shares 88 and 89% identity with mouse and rat Annexin A1, respectively.
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