|Detection of Human/Mouse/Rat APE by Western Blot. Western blot shows lysates of NRK rat normal kidney cell line, Raji human Burkitt's lymphoma cell line, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat APE Monoclonal Antibody (Catalog # MAB1044) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for APE at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Human APE (also known as Ref-1) is the apurinic/apyrimidinic (AP) endonuclease required for efficient DNA base excision repair (BER). Following the removal of a damaged base by a DNA glycosylase, APE cleaves the AP site to allow resynthesis and ligation to complete repair. In addition, APE/Ref-1 acts as a factor that regulates the redox state of multiple transcription factors, including c-Jun, c-Fos, NF‑ kappa B, and p53.
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