Human/Mouse/Rat CDK2 Antibody

Catalog # Availability Size / Price Qty
AF4654
AF4654-SP
Detection of Human/Mouse CDK2 by Western Blot.
5 Images
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse/Rat CDK2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat CDK2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) CDK4 and rhCDK6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CDK2
Met1-Leu298
Accession # P24941
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
3-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human/Mouse CDK2 antibody by Western Blot. View Larger

Detection of Human/Mouse CDK2 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human CDK2, CDK4, and CDK6 (5 ng/lane) were included. A specific band for CDK2 was detected at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Rat CDK2 antibody by Western Blot. View Larger

Detection of Rat CDK2 by Western Blot. Western blot shows lysates of C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CDK2 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry CDK2 antibody in K562 Human Cell Line by Immunocytochemistry (ICC). View Larger

CDK2 in K562 Human Cell Line. CDK2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry CDK2 antibody in 3T3-L1 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

CDK2 in 3T3‑L1 Mouse Cell Line. CDK2 was detected in immersion fixed 3T3-L1 mouse embryonic fibroblast adipose-like cell line using Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry CDK2 antibody in Human Lung Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

CDK2 in Human Lung Cancer Tissue. CDK2 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cancer cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDK2

CDK2 (cyclin-dependent kinase 2), a serine/threonine protein kinase, is the catalytic subunit of the heterodimeric cyclin-dependent kinase complex required for G1/S phase transition. The kinase activity of CDK2 is regulated by the association with a cyclin subunit, CDK inhibitors, and its phosphorylation state. The active CDK2/cyclin A complex interacts with the N-terminus of E2F-1 and directs the phosphorylation of E2F-1 and DP-1. The active CDK2/cyclin E complex phosphorylates Rb which disrupts its binding to E2F, allowing E2F activation and transcription of the genes necessary for S-phase entry and progression. Inhibition of the CDK2/cyclin complex can be attributed to its association with p21Waf1/Cip1 and p27Kip1 and the phosphorylation of CDK2 on Thr14 and Tyr15. The activation of the CDK2/cyclin complex requires the phosphorylation of Thr160 and the dephosphorylation of Tyr14 and Tyr15.

Long Name
Cyclin-dependent Kinase 2
Entrez Gene IDs
1017 (Human); 12566 (Mouse); 362817 (Rat)
Alternate Names
cdc2-related protein kinase; CDK2; cell devision kinase 2; Cell division protein kinase 2; cyclin-dependent kinase 2; EC 2.7.11; EC 2.7.11.22; p33 protein kinase; p33(CDK2)

Product Datasheets

Citations for Human/Mouse/Rat CDK2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Cisplatin exposure causes c-Myc-dependent resistance to CDK4/6 inhibition in HPV-negative head and neck squamous cell carcinoma
    Authors: AM Robinson, R Rathore, NJ Redlich, DR Adkins, T VanArsdale, BA Van Tine, LS Michel
    Cell Death Dis, 2019;10(11):867.
    Species: Xenograft
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Chemotherapy-induced differential cell cycle arrest in B cell lymphomas affects their sensitivity to Wee1 inhibition
    Authors: X Wang, Z Chen, AK Mishra, A Silva, W Ren, Z Pan, JH Wang
    Haematologica, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat CDK2 Antibody

Average Rating: 4 (Based on 1 Review)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human/Mouse/Rat CDK2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse/Rat CDK2 Antibody
By Anonymous on 01/25/2018
Application: WB Sample Tested: PC-3 human prostate cancer cell line Species: Human