Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CDK2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CDK2
Met1-Leu298
Accession # P24941

Specificity

Detects human, mouse, and rat CDK2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) CDK4 and rhCDK6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat CDK2 Antibody

Detection of Human/Mouse CDK2 antibody by Western Blot.

Detection of Human/Mouse CDK2 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). For additional reference, recombinant human CDK2, CDK4, and CDK6 (5 ng/lane) were included. A specific band for CDK2 was detected at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Rat CDK2 antibody by Western Blot.

Detection of Rat CDK2 by Western Blot.

Western blot shows lysates of C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for CDK2 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CDK2 antibody in K562 Human Cell Line by Immunocytochemistry (ICC).

CDK2 in K562 Human Cell Line.

CDK2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CDK2 antibody in 3T3-L1 Mouse Cell Line by Immunocytochemistry (ICC).

CDK2 in 3T3‑L1 Mouse Cell Line.

CDK2 was detected in immersion fixed 3T3-L1 mouse embryonic fibroblast adipose-like cell line using Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

CDK2 antibody in Human Lung Cancer Tissue by Immunohistochemistry (IHC-P).

CDK2 in Human Lung Cancer Tissue.

CDK2 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cancer cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of CDK2 by Western Blot

Detection of CDK2 by Western Blot

LPS suppressed EPCs cell viability via inducing cell pyroptosis. (a, b) NLRP3 was silenced in EPCs cells. (c) MTT assay was used to examine cell viability. (d) The expression status of Cyclin D1 and CDK2 were determined by Western blot analysis. Individual experiment repeated 3 times, and *P < 0.05 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34738867), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human/Mouse/Rat CDK2 by Simple WesternTM.

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for CDK2 at approximately 38 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat CDK2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line and immersion fixed 3T3-L1 mouse embryonic fibroblast adipose-like cell line

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung cancer tissue

Simple Western

20 µg/mL
Sample: Lysates of K562 human chronic myelogenous leukemia cell line and HEK293T human embryonic kidney cell line.

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line

Reviewed Applications

Read 1 review rated 4 using AF4654 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CDK2

CDK2 (cyclin-dependent kinase 2), a serine/threonine protein kinase, is the catalytic subunit of the heterodimeric cyclin-dependent kinase complex required for G1/S phase transition. The kinase activity of CDK2 is regulated by the association with a cyclin subunit, CDK inhibitors, and its phosphorylation state. The active CDK2/cyclin A complex interacts with the N-terminus of E2F-1 and directs the phosphorylation of E2F-1 and DP-1. The active CDK2/cyclin E complex phosphorylates Rb which disrupts its binding to E2F, allowing E2F activation and transcription of the genes necessary for S-phase entry and progression. Inhibition of the CDK2/cyclin complex can be attributed to its association with p21Waf1/Cip1 and p27Kip1 and the phosphorylation of CDK2 on Thr14 and Tyr15. The activation of the CDK2/cyclin complex requires the phosphorylation of Thr160 and the dephosphorylation of Tyr14 and Tyr15.

Long Name

Cyclin-dependent Kinase 2

Alternate Names

cdc2-related protein kinase, cell devision kinase 2, Cell division protein kinase 2, cyclin-dependent kinase 2, EC 2.7.11, EC 2.7.11.22, p33 protein kinase, p33(CDK2)

Entrez Gene IDs

1017 (Human); 12566 (Mouse); 362817 (Rat)

Gene Symbol

CDK2

UniProt

P24941

Additional CDK2 Products

Product Documents for Human/Mouse/Rat CDK2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat CDK2 Antibody

For research use only

Related Research Areas

Cell Cycle
Intracellular Kinases

Citations for Human/Mouse/Rat CDK2 Antibody

Customer Reviews for Human/Mouse/Rat CDK2 Antibody (1)

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  • Human/Mouse/Rat CDK2 Antibody
    Name: Thiruloga Chandru
    Application: Western Blot
    Sample Tested: PC-3 human prostate cancer cell line
    Species: Human
    Verified Customer | Posted 01/25/2018
    Human/Mouse/Rat CDK2 Antibody AF4654

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