Human/Mouse/Rat Contactin-1 Antibody

(6 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human Contactin-1 in direct ELISAs.  Detects human, mouse, and rat Contactin-1 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Contactin-1
    Glu21-Ser993
    Accession # CAA79696
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize Contactin‑1-mediated adhesion of the C6 rat glioma cell line [Revest, J.M. et al. (1999) Adv. Exp. Med. Biol. 468:309]. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 2 µg/mL Recombinant Human Contactin‑1 Fc Chimera.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Contactin‑1 in Human Dorsal Root Ganglia. Contactin‑1 was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglia using 15 µg/mL Goat Anti-Human/
Mouse/Rat Contactin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human, Mouse, and Rat Contactin‑1 by Western Blot. Western blot shows lysates of human brain (cortex) tissue, human brain (cerebellum) tissue, mouse brain (cerebellum) tissue, mouse brain (total) tissue, and rat brain (total) tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/
Rat Contactin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Contactin‑1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Mouse Contactin‑1 by Simple WesternTM. Simple Western lane view shows lysates of human brain tissue and mouse brain tissue, loaded at 0.2 mg/mL. A specific band was detected for Contactin‑1 at approximately 158 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Contactin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Cell Adhesion Mediated by Contactin‑1 and Neutralization by Human Contactin‑1 Antibody. Recombinant Human Contactin‑1 Fc Chimera (Catalog # 904-CN), immobilized onto a microplate, supports the adhesion of the C6 rat glioma cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Contactin‑1 Fc Chimera (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat
Contactin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904). The ND50 is typically 1-5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Contactin-1

Contactin-1 (CNTN1) is a member of the contactin subgroup within the immunoglobulin superfamily. It was originally designated contactin in human, F3 in rodents, and F11 in chicken. Other members of this family in human include Contactin-2 (TAG-1), Contactin-5 (NB-2), and Contactin-6 (NB-3). Additional family members have been described in other species. CNTN1 shares less than 50% amino acid sequence identity with the other contactins. The human and rodent CNTN1 proteins share 96% sequence identity. The 998 amino acid mature protein contains 6 Ig-like domains and 4 fibronectin type III-like domains, and is attached to the membrane by a GPI anchor. CNTN1 is differentially expressed in numerous neuronal tissues and functions in nervous system development. It associates with two other cell-surface proteins believed to participate in signal transduction, receptor protein tyrosine phosphatase beta (RPTP beta ) and Contactin-associated protein (Caspr). Reported ligands include Nr-CAM and the extracellular matrix glycoprotein, tenascin.

  • References:
    1. Reid, R.A. et al. (1994) Brain Res. Mol. Brain Res. 21:1.
    2. Walsh, F.S. and P. Doherty (1991) Cell Biol. Int. Rep. 15:1151.
  • Entrez Gene IDs:
    1272 (Human); 12805 (Mouse)
  • Alternate Names:
    CNTN1; contactin 1; Contactin1; Contactin-1; F11; F3; F3cam; Glycoprotein gp135; GP135; Neural cell surface protein F3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Contactin-1 regulates myelination and nodal/paranodal domain organization in the central nervous system.
    Authors: Colakoglu G, Bergstrom-Tyrberg U, Berglund E, Ranscht B
    Proc Natl Acad Sci U S A, 2014;111(3):E394-403.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Lipid raft-dependent endocytosis of close homolog of adhesion molecule L1 (CHL1) promotes neuritogenesis.
    Authors: Tian N, Leshchyns'ka I, Welch J, Diakowski W, Yang H, Schachner M, Sytnyk V
    J Biol Chem, 2012;287(53):44447-63.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Lewis(x) and alpha2,3-sialyl glycans and their receptors TAG-1, Contactin, and L1 mediate CD24-dependent neurite outgrowth.
    Authors: Lieberoth A, Splittstoesser F, Katagihallimath N, Jakovcevski I, Loers G, Ranscht B, Karagogeos D, Schachner M, Kleene R
    J. Neurosci., 2009;29(20):6677-90.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: IP
  4. Versican V2 assembles the extracellular matrix surrounding the nodes of ranvier in the CNS.
    Authors: Dours-Zimmermann MT, Maurer K, Rauch U, Stoffel W, Fassler R, Zimmermann DR
    J. Neurosci., 2009;29(24):7731-42.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Knockdown of contactin-1 expression suppresses invasion and metastasis of lung adenocarcinoma.
    Authors: Su JL, Yang CY, Shih JY, Wei LH, Hsieh CY, Jeng YM, Wang MY, Yang PC, Kuo ML
    Cancer Res., 2006;66(5):2553-61.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Contactin associates with sodium channel Nav1.3 in native tissues and increases channel density at the cell surface.
    Authors: Shah BS, Rush AM, Liu S, Tyrrell L, Black JA, Dib-Hajj SD, Waxman SG
    J. Neurosci., 2004;24(33):7387-99.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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