Human/Mouse/Rat Contactin-1 Antibody

Name: Human/Mouse/Rat Contactin-1 Antibody
Brand: R&D Systems

(8 citations)

Datasheet / CoA / SDS

Contactin‑1 in Human Dorsal Root Ganglia.

Immunohistochemistry

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Western Blot

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Simple Western

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Neutralization

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Product Details

Citations (8)

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Species Reactivity

Human, Mouse, Rat
Specificity

Detects human Contactin-1 in direct ELISAs. Detects human, mouse, and rat Contactin-1 in Western blots.
Source

Polyclonal Goat IgG
Purification

Antigen Affinity-purified
Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Contactin-1
Glu21-Ser993
Accession # CAA79696
Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.
Label

Unconjugated

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

0.5 µg/mL

See below
Simple Western

5 µg/mL

See below
Immunohistochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize Contactin‑1-mediated adhesion of the C6 rat glioma cell line [Revest, J.M. et al. (1999) Adv. Exp. Med. Biol. 468:309]. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 2 µg/mL Recombinant Human Contactin‑1 Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry click image to view larger	Contactin‑1 in Human Dorsal Root Ganglia. Contactin‑1 was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglia using 15 µg/mL Goat Anti-Human/ Mouse/Rat Contactin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western Blot click image to view larger	Detection of Human, Mouse, and Rat Contactin‑1 by Western Blot. Western blot shows lysates of human brain (cortex) tissue, human brain (cerebellum) tissue, mouse brain (cerebellum) tissue, mouse brain (total) tissue, and rat brain (total) tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/ Rat Contactin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Contactin‑1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western click image to view larger	Detection of Human and Mouse Contactin‑1 by Simple Western^TM. Simple Western lane view shows lysates of human brain tissue and mouse brain tissue, loaded at 0.2 mg/mL. A specific band was detected for Contactin‑1 at approximately 158 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Contactin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Neutralization click image to view larger	Cell Adhesion Mediated by Contactin‑1 and Neutralization by Human Contactin‑1 Antibody. Recombinant Human Contactin‑1 Fc Chimera (Catalog # 904-CN), immobilized onto a microplate, supports the adhesion of the C6 rat glioma cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Contactin‑1 Fc Chimera (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Contactin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF904). The ND₅₀ is typically 1-5 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Contactin-1

Contactin-1 (CNTN1) is a member of the contactin subgroup within the immunoglobulin superfamily. It was originally designated contactin in human, F3 in rodents, and F11 in chicken. Other members of this family in human include Contactin-2 (TAG-1), Contactin-5 (NB-2), and Contactin-6 (NB-3). Additional family members have been described in other species. CNTN1 shares less than 50% amino acid sequence identity with the other contactins. The human and rodent CNTN1 proteins share 96% sequence identity. The 998 amino acid mature protein contains 6 Ig-like domains and 4 fibronectin type III-like domains, and is attached to the membrane by a GPI anchor. CNTN1 is differentially expressed in numerous neuronal tissues and functions in nervous system development. It associates with two other cell-surface proteins believed to participate in signal transduction, receptor protein tyrosine phosphatase beta (RPTP beta ) and Contactin-associated protein (Caspr). Reported ligands include Nr-CAM and the extracellular matrix glycoprotein, tenascin.

References:
1. Reid, R.A. et al. (1994) Brain Res. Mol. Brain Res. 21:1.
2. Walsh, F.S. and P. Doherty (1991) Cell Biol. Int. Rep. 15:1151.

Entrez Gene IDs:

1272 (Human); 12805 (Mouse)

Alternate Names:

CNTN1; contactin 1; Contactin1; Contactin-1; F11; F3; F3cam; Glycoprotein gp135; GP135; Neural cell surface protein F3

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species	Applications	Sample Type
Antibodies against peripheral nerve antigens in chronic inflammatory demyelinating polyradiculoneuropathy Authors: L Querol, AM Siles, R Alba-Rovir, A Jáuregui, J Devaux, C Faivre-Sar, J Araque, R Rojas-Garc, J Diaz-Maner, E Cortés-Vic, G Nogales-Ga, M Navas-Madr, E Gallardo, I Illa Sci Rep, 2017;7(1):14411. Species: Human Sample Type: Whole Cells Application: ICC Schwannomin-interacting protein 1 isoform IQCJ-SCHIP1 is a multi-partner ankyrin and spectrin binding protein involved in the organization of nodes of Ranvier Authors: Pierre-Marie Martin J. Biol. Chem, 2016;0(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC Contactin-1 regulates myelination and nodal/paranodal domain organization in the central nervous system. Authors: Colakoglu G, Bergstrom-Tyrberg U, Berglund E, Ranscht B Proc Natl Acad Sci U S A, 2014;111(3):E394-403. Species: Mouse Sample Type: Tissue Homogenates Application: WB Lipid raft-dependent endocytosis of close homolog of adhesion molecule L1 (CHL1) promotes neuritogenesis. Authors: Tian N, Leshchyns'ka I, Welch J, Diakowski W, Yang H, Schachner M, Sytnyk V J Biol Chem, 2012;287(53):44447-63. Species: Mouse Sample Type: Tissue Homogenates Application: WB Lewis(x) and alpha2,3-sialyl glycans and their receptors TAG-1, Contactin, and L1 mediate CD24-dependent neurite outgrowth. Authors: Lieberoth A, Splittstoesser F, Katagihallimath N, Jakovcevski I, Loers G, Ranscht B, Karagogeos D, Schachner M, Kleene R J. Neurosci., 2009;29(20):6677-90. Species: Mouse Sample Type: Tissue Homogenates Application: IP Versican V2 assembles the extracellular matrix surrounding the nodes of ranvier in the CNS. Authors: Dours-Zimmermann MT, Maurer K, Rauch U, Stoffel W, Fassler R, Zimmermann DR J. Neurosci., 2009;29(24):7731-42. Species: Mouse Sample Type: Whole Tissue Application: IHC Knockdown of contactin-1 expression suppresses invasion and metastasis of lung adenocarcinoma. Authors: Su JL, Yang CY, Shih JY, Wei LH, Hsieh CY, Jeng YM, Wang MY, Yang PC, Kuo ML Cancer Res., 2006;66(5):2553-61. Species: Human Sample Type: Cell Lysates Application: WB Contactin associates with sodium channel Nav1.3 in native tissues and increases channel density at the cell surface. Authors: Shah BS, Rush AM, Liu S, Tyrrell L, Black JA, Dib-Hajj SD, Waxman SG J. Neurosci., 2004;24(33):7387-99. Species: Human Sample Type: Whole Cells Application: ICC

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Human/Mouse/Rat Contactin-1 Antibody

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FAQs

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

Antigen Retrieval Reagent-Universal

Antigen Retrieval Reagent-Basic

Mounting Medium

Antigen Retrieval Reagent Sampler (50 mL each)

Antigen Retrieval Reagent-Acidic

VisUCyte Antigen Retrieval Reagent-Universal

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NorthernLights Guard Mounting Media

Normal Goat IgG Control

Reconstitution Buffer 1 (PBS)

Goat IgG HRP-conjugated Antibody

Goat IgG HRP-conjugated Antibody

Goat IgG (H+L) PE-conjugated Antibody

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Goat IgG VisUCyte HRP Polymer

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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