Cytochrome c Antibody (2.7D5)

R&D Systems | Catalog # MAB899

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 2.7D5
View all Cytochrome c Primary Antibodies »

Product Specifications

Immunogen

Pigeon Cytochrome c

Specificity

Detects native human, mouse, and rat Cytochrome c.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Cytochrome c Antibody (2.7D5)

Immunoprecipitation of Human/Mouse Cytochrome c.

Immunoprecipitation of Human/Mouse Cytochrome c.

Cytochrome c was immuno-precipitated from lysates (5 x 106cells) of Jurkat human acute T cell leukemia and CTLL-2 mouse cytotoxic T cell line following incubation with 5 µg Mouse Anti-Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB899) for 1 hour at 4 °C. Cytochrome c-antibody comp-lexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immunoprecip-itated Cytochrome c (left panel) was detected by Western blot using 0.5 µg/mL Mouse Anti-Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). For additional reference, Western blot (right panel) shows lysates of Jurkat and CTLL2 cell lines probed with 15 µg/mL Mouse Anti-Human/ Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). View our recommended buffer recipes for immunoprecipitation.

Applications for Cytochrome c Antibody (2.7D5)

Application
Recommended Usage

Immunoprecipitation

1 µg/106 cells
Sample: Jurkat human acute T cell leukemia cell line and CTLL‑2 mouse cytotoxic T cell line, see our available Western blot detection antibodies

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytochrome c

Cytochrome c is a critical mitochondrial outer membrane-associated protein in the electron transport chain (1).  Reduction and oxidation of an iron molecule (Fe3+ to Fe2+ and back) within its central heme group allow it to receive an electron from the cytochrome c1 subunit of cytochrome reductase and pass it to cytochrome a within the cytochrome oxidase complex (1).  Release of cytochrome c from mitochondria occurs during initiation of apoptotic pathways, either by mechanisms that rupture or that do not rupture the mitochondial membrane (2, 3).  Once in the cytosol, cytochrome c forms a complex with procaspase 9, recruiting Apaf-1 (apoptotic protease activating factor-1) and dATP to form apoptosomes, which activate caspase and ultimately destroy the cell (2, 3).  Cytochrome c is highly conserved, with 90% or greater amino acid identity among human, mouse, rat, cow and dog sequences (4).  After cytochrome c translation, the initial methionine is cleaved to form the 104 amino acid mature form.

References

  1. Zubay, G. L., 1983, “Biochemistry”, pp. 380, Addison-Wesley Pub. Co, Inc.
  2. Liu, X. et al., 1996, Cell 86:147.
  3. Li, P. et al., 1997, Cell 91:479.
  4. Matsubara, H and E. L. Smith, 1963, J. Biol. Chem. 238:2732.

Alternate Names

CYCS

Entrez Gene IDs

54205 (Human)

Gene Symbol

CYCS

Additional Cytochrome c Products

Product Documents for Cytochrome c Antibody (2.7D5)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Cytochrome c Antibody (2.7D5)

For research use only

Related Research Areas

Bcl-2 Proteins and Regulators

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Protocols

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