Human/Mouse/Rat Cytochrome c Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects native human, mouse, and rat Cytochrome c.
  • Source
    Monoclonal Mouse IgG2A Clone # 2.7D5
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Pigeon Cytochrome c
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunoprecipitation
    1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunoprecipitation
Immunoprecipitation of Human/Mouse Cytochrome c. Cytochrome c was immuno­precipitated from lysates (5 x 106 cells) of Jurkat human acute T cell leukemia and CTLL‑2 mouse cytotoxic T cell line following incubation with 5 µg Mouse Anti-Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB899) for 1 hour at 4 °C. Cytochrome c-antibody comp­lexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immunoprecip­itated Cytochrome c (left panel) was detected by Western blot using 0.5 µg/mL Mouse Anti-Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). For additional reference, Western blot (right panel) shows lysates of Jurkat and CTLL2 cell lines probed with 15 µg/mL Mouse Anti-Human/
Mouse/Rat Cytochrome c Monoclonal Antibody (Cata­log # MAB897). View our recommended buffer recipes for immunoprecipitation.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytochrome c
Cytochrome c is a critical mitochondrial outer membrane-associated protein in the electron transport chain (1).  Reduction and oxidation of an iron molecule (Fe3+ to Fe2+ and back) within its central heme group allow it to receive an electron from the cytochrome c1 subunit of cytochrome reductase and pass it to cytochrome a within the cytochrome oxidase complex (1).  Release of cytochrome c from mitochondria occurs during initiation of apoptotic pathways, either by mechanisms that rupture or that do not rupture the mitochondial membrane (2, 3).  Once in the cytosol, cytochrome c forms a complex with procaspase 9, recruiting Apaf-1 (apoptotic protease activating factor-1) and dATP to form apoptosomes, which activate caspase and ultimately destroy the cell (2, 3).  Cytochrome c is highly conserved, with 90% or greater amino acid identity among human, mouse, rat, cow and dog sequences (4).  After cytochrome c translation, the initial methionine is cleaved to form the 104 amino acid mature form.
  • References:
    1. Zubay, G. L., 1983, “Biochemistry”, pp. 380, Addison-Wesley Pub. Co, Inc.
    2. Liu, X. et al., 1996, Cell 86:147.
    3. Li, P. et al., 1997, Cell 91:479.
    4. Matsubara, H and E. L. Smith, 1963, J. Biol. Chem. 238:2732.
  • Entrez Gene IDs:
    54205 (Human)
  • Alternate Names:
    CYCHCS; CYCS; Cytochrome c; cytochrome c, somatic; THC4
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