Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 7H8.2C12
View all Cytochrome c Primary Antibodies »

Product Specifications

Immunogen

Pigeon Cytochrome c

Specificity

Reacts with denatured human, mouse and rat Cytochrome c, but not native Cytochrome c in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat Cytochrome c Antibody

Detection of Human and Mouse Cytochrome c antibody by Western Blot.

Detection of Human and Mouse Cytochrome c by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytochrome c at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Detection of Human Cytochrome c antibody by Simple WesternTM.

Detection of Human Cytochrome c by Simple WesternTM.

Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Cytochrome c at approximately 23 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human/Mouse/Rat Cytochrome c Monoclonal Antibody (Catalog # MAB897). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human Cytochrome c by Western Blot

Detection of Human Cytochrome c by Western Blot

FAM210B is transported into and is localized in the mitochondria. (a) Schematic representation of FAM210B domains based on the primary structure of FAM210B. (b) Confocal microscopy of HeLa cells transfected with GFP-tagged human FAM210B and RFP-tagged mitochondria. FAM210B-GFP, GFP sequence introduced at the C terminus of FAM210B; FAM210B (MTS)-GFP, the MTS (aa 1–47) of FAM210B was added to the GFP N terminus; FAM210B ( delta MTS)-GFP, GFP was added to the C terminus of MTS (1–47)-deleted CRIF1. Scale bar, 20 mm. (c) Confocal microscopy of HeLa cells transfected with GFP-tagged human FAM210B and RFP-tagged endoplasmic reticulum. (d) Western blotting analysis following subcellular fractionation of GFP-tagged human FAM210B HeLa cells. (e) Western blotting analysis following subcellular fractionation of endogenous in HeLa cells. (f) The mitochondria of HeLa cells were swollen and sonicated to disrupt membranes, washed with alkali buffer (pH 11.5) to detach loosely associated proteins from membranes, and then re-isolated by ultracentrifugation. The supernatant (Supe) and membrane fractions (Pellet) were subjected to western blotting for FAM210B, TOM20, or MnSOD. (g) Mitochondria isolated from HeLa cells were subjected to proteinase K (PK) proteolysis to digest exposed proteins, and detergent (SDS) was used to disrupt both IMMs (inner membrane of mitochondria) and OMMs (outer membrane of mitochondria). The lysates were resolved and subjected to immunoblot analyses. The submitochondrial markers used are Tom20 (OMM), Cyt C (Cytochrome c, intermembrane space), NDUFS1 (IMM), and MnSOD (mitochondrial matrix) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28594398), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat Cytochrome c Antibody

Application
Recommended Usage

Simple Western

2.5 µg/mL
Sample: Human heart tissue

Western Blot

0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and CTLL-2 mouse cytotoxic T cell line

Reviewed Applications

Read 1 review rated 5 using MAB897 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytochrome c

Cytochrome c is a mitochondrial electron transport protein that functions in oxidative phosphorylation. Release of Cytochrome c into the cytosol is an early step in apoptotic cell death.

Alternate Names

CYCS

Entrez Gene IDs

54205 (Human)

Gene Symbol

CYCS

Additional Cytochrome c Products

Product Documents for Human/Mouse/Rat Cytochrome c Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Cytochrome c Antibody

For research use only

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Human/Mouse/Rat Cytochrome c Antibody

Customer Reviews for Human/Mouse/Rat Cytochrome c Antibody (1)

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  • Human/Mouse/Rat Cytochrome c Antibody
    Name: Joseph Moore
    Application: Western Blot
    Sample Tested: Cartilage tissue
    Species: Mouse
    Verified Customer | Posted 08/06/2019
    Antibody Dilution 1:100. We used this on mouse meniscus lysates. Antibody was incubated overnight and developed with ECL kit. It worked very well and we would use it again.
    Human/Mouse/Rat Cytochrome c Antibody MAB897

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