Human/Mouse/Rat ERK1/ERK2 Antibody

Catalog # Availability Size / Price Qty
MAB15761
MAB15761-SP
Detection of Human, Mouse, and Rat ERK1/ERK2 by Western Blot.
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Product Details
Citations (3)
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Human/Mouse/Rat ERK1/ERK2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human ERK1 and ERK2 in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant human (rh) ERK2 and no cross-reactivity with rhMAPK4, 6, 7, 9, 10, 11, or 12 is observed.
Source
Monoclonal Mouse IgG2B Clone # 631122
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human ERK1 synthetic peptide
KKISPFEHQTYCQRTLR
Accession # P27361 (ERK1) & P28482 (ERK2)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat ERK1/ERK2 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat ERK1/ERK2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, BaF3 mouse pro-B cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB15761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for ERK1/ERK2 at approximately 42 and 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERK1/ERK2

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2.

ERK5, also known as Big Mitogen-activated Protein Kinase 1 (BMK1) and MAPK7, is activated by several mechanisms, including receptor tyrosine kinases, G protein-coupled receptors, and osmotic stress. Like ERK1 and ERK2, ERK5 contains the conserved Thr-Glu-Tyr activation motif in its activation loop. Unlike these ERKs, however, ERK5 contains a unique C-terminal domain that regulates its activation and nuclear translocation.

Long Name
Extracellular Signal-regulated Kinase 1/2
Alternate Names
ERK1/ERK2

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Citations for Human/Mouse/Rat ERK1/ERK2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Rnd3 Is a Crucial Mediator of the Invasive Phenotype of Glioblastoma Cells Downstream of Receptor Tyrosine Kinase Signalling
    Authors: B Almarán, G Ramis, S Fernández, P Villalonga
    Cells, 2022-11-22;11(23):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. An engineered IL-2 partial agonist promotes CD8+ T cell stemness
    Authors: F Mo, Z Yu, P Li, J Oh, R Spolski, L Zhao, CR Glassman, TN Yamamoto, Y Chen, FM Golebiowsk, D Hermans, S Majri-Morr, LK Picton, W Liao, M Ren, X Zhuang, S Mitra, JX Lin, L Gattinoni, JD Powell, NP Restifo, KC Garcia, WJ Leonard
    Nature, 2021-09-15;597(7877):544-548.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Pharmaceutical immunoglobulin G impairs anti-carcinoma activity of oxaliplatin in colon cancer cells
    Authors: Y Shang, X Zhang, L Lu, K Jiang, M Krohn, S Matschos, CS Mullins, B Vollmar, D Zechner, P Gong, M Linnebache
    British Journal of Cancer, 2021-02-09;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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