Human/Mouse/Rat Fc mu R/FAIM3 Antibody

Catalog # Availability Size / Price Qty
MAB9494-100
MAB9494-SP
Detection  of Human, Mouse, and Rat Fc mu R/FAIM3 by Western Blot.
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Human/Mouse/Rat Fc mu R/FAIM3 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Fc mu R/FAIM3 in direct ELISAs. Detects human, mouse, and rat Fc mu R/FAIM3 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 992338
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived recombinant human Fc mu R/FAIM3
Arg18-Gly251
Accession # O60667
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human, Mouse, and Rat Fc mu R/FAIM3 by Western Blot. View Larger

Detection of Human, Mouse, and Rat Fc mu R/FAIM3 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, A20 mouse B cell lymphoma cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Fc mu R/FAIM3 Monoclonal Antibody (Catalog # MAB9494) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fc mu R/FAIM3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of FAIM3 in HEK293 Human Cell Line Transfected with Human FAIM3 and eGFP by Flow Cytometry. View Larger

Detection of FAIM3 in HEK293 Human Cell Line Transfected with Human FAIM3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human FAIM3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human/Mouse/Rat FAIM3 Monoclonal Antibody (Catalog # MAB9494) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody(Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc mu R/FAIM3

The human FAIM3 gene (also known as FCMR or TOSO), is a transmembrane sialoglycoprotein expressed mainly by lymphocytes. FAIM3 is a type I membrane protein with an intracellular C-terminal domain and an extracellular N-terminal domain (1). The extracellular domain has homology to the immunoglobulin variable region domains (1) and FAIM3 is identified as an Fc receptor for IgM (2, 3). The amino acid sequence of human FAIM3 is 58% and 55% identical to that of mouse and rat FAIM3, respectively. FAIM3 was shown to be over-expressed in chronic lymphocytic leukemia (CLL) (4) and associated with disease progression (5, 6). FAIM3 has also been linked to the homeostasis and activation of the innate immune system (7). Interestingly, there is growing evidence that neurodegenerative diseases are associated with the activation of the immune surveillance system. This system is responsible for controlling danger signals and responding accordingly to the magnitude and duration of the threat (8, 9).

References
  1. Song, Y. et al (2005) J. Biol. Chem. 280(10):9618.
  2. Shima H et al. (2010) Int. Immunol. 22:149.
  3. Kubagawa H et al. (2009) J. Exp. Med. 206:2779.
  4. Proto-Siqueira R et al. (2008) Blood, 112:394.
  5. Pallasch, C et al. (2008) Blood, 112:4213.
  6. Pallasch, C P et al (2009) Leukemia & lymphoma, 50:498.
  7. Sigruener, A et al (2007) Biochemical and biophysical research communications, 359:723.
  8. Richards R I et al (2016) Front. Neurosci. 10:193.
  9. Planells-Ferrer L et al (2016) J Neurochem. 139:11.
Long Name
Fc mu Receptor
Entrez Gene IDs
9214 (Human); 69169 (Mouse); 548326 (Rat)
Alternate Names
FAIM3; Fas apoptotic inhibitory molecule 3; Fc mu R; Fc mu receptor; FCMR; immunoglobulin mu Fc receptor; Regulator of Fas-induced apoptosis Toso; Toso; TOSOIgM Fc receptor

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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