Human/Mouse/Rat Gelsolin/GSN Antibody Summary
Accession # P06396
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat Gelsolin/GSN by Western Blot. Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line, MEF mouse embryonic feeder cells, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Gelsolin/GSN at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Gelsolin/GSN in Human Kidney. Gelsolin/GSN was detected in formalin fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli and distal convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Gelsolin/GSN by Simple WesternTM. Simple Western lane view shows lysates of SK‑Mel‑28 human malignant melanoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Gelsolin/GSN at approximately 96 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Gelsolin, also known as GSN, actin-depolymerizing factor/ADF, AGEL and Brevin, is a 90-95 kDa member of the villin/gelsolin family. Widely expressed, Gelsolin binds to actin and fibronectin, and is found both secreted in plasma and in cytoplasm. Cytoplasmic Gelsolin lacks 51 N-terminal amino acids (aa) present in the secreted protein. Gelsolin was identified by its ability to sever actin filaments in the presence of submicromolar calcium, and plays a role in ciliogenesis. Defects in Gelsolin cause amyloidosis type 5, a hereditary disease characterized by gelsolin amyloid deposition. Full-length human Gelsolin is 782 aa and shares 93% aa identity with mouse and rat Gelsolin.
Citation for Human/Mouse/Rat Gelsolin/GSN Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Lower Expression of Gelsolin in Colon Cancer and Its Diagnostic Value in Colon Cancer Patients
Authors: Z Chen, K Li, X Yin, H Li, Y Li, Q Zhang, H Wang, Y Qiu
J Cancer, 2019;10(5):1288-1296.
Sample Types: Whole Cells
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