Detection of Human, Mouse, and Rat Gelsolin/GSN by Western Blot. Western blot shows lysates of SK‑Mel‑28 human malignant melanoma cell line, MEF mouse embryonic feeder cells, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Gelsolin/GSN at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Gelsolin/GSN in Human Kidney. Gelsolin/GSN was detected in formalin fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli and distal convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Gelsolin/GSN by Simple WesternTM. Simple Western lane view shows lysates of SK‑Mel‑28 human malignant melanoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Gelsolin/GSN at approximately 96 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Gelsolin, also known as GSN, actin-depolymerizing factor/ADF, AGEL and Brevin, is a 90-95 kDa member of the villin/gelsolin family. Widely expressed, Gelsolin binds to actin and fibronectin, and is found both secreted in plasma and in cytoplasm. Cytoplasmic Gelsolin lacks 51 N-terminal amino acids (aa) present in the secreted protein. Gelsolin was identified by its ability to sever actin filaments in the presence of submicromolar calcium, and plays a role in ciliogenesis. Defects in Gelsolin cause amyloidosis type 5, a hereditary disease characterized by gelsolin amyloid deposition. Full-length human Gelsolin is 782 aa and shares 93% aa identity with mouse and rat Gelsolin.
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