Human/Mouse/Rat Gelsolin/GSN Antibody

Catalog # Availability Size / Price Qty
MAB8170
MAB8170-SP
Human/Mouse/Rat Gelsolin/GSN Antibody in Western Blot
3 Images
Product Details
Citations (1)
FAQs
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Human/Mouse/Rat Gelsolin/GSN Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Gelsolin/GSN in ELISAs. Detects human, mouse and rat Gelsolin/GSN in Western Blots.
Source
Monoclonal Mouse IgG1 Clone # 893205
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human Gelsolin/GSN
Met1-Ala782
Accession # P06396
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat Gelsolin/GSN by Western Blot. View Larger

Detection of Human, Mouse, and Rat Gelsolin/GSN by Western Blot. Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line, MEF mouse embryonic feeder cells, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Gelsolin/GSN at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Gelsolin/GSN in Human Kidney. View Larger

Gelsolin/GSN in Human Kidney. Gelsolin/GSN was detected in formalin fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli and distal convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human Gelsolin/GSN by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Gelsolin/GSN by Simple WesternTM. Simple Western lane view shows lysates of SK‑Mel‑28 human malignant melanoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Gelsolin/GSN at approximately 96 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse/Rat Gelsolin/GSN Monoclonal Antibody (Catalog # MAB8170). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Gelsolin/GSN

Gelsolin, also known as GSN, actin-depolymerizing factor/ADF, AGEL and Brevin, is a 90-95 kDa member of the villin/gelsolin family.  Widely expressed, Gelsolin binds to actin and fibronectin, and is found both secreted in plasma and in cytoplasm.  Cytoplasmic Gelsolin lacks 51 N-terminal amino acids (aa) present in the secreted protein.  Gelsolin was identified by its ability to sever actin filaments in the presence of submicromolar calcium, and plays a role in ciliogenesis.  Defects in Gelsolin cause amyloidosis type 5, a hereditary disease characterized by gelsolin amyloid deposition.  Full-length human Gelsolin is 782 aa and shares 93% aa identity with mouse and rat Gelsolin.

Entrez Gene IDs
2934 (Human); 227753 (Mouse); 296654 (Rat)
Alternate Names
Actin-depolymerizing factor; ADF; AGEL; Brevin; DKFZp313L0718; gelsolin (amyloidosis, Finnish type); Gelsolin; GSN

Product Datasheets

Citation for Human/Mouse/Rat Gelsolin/GSN Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Lower Expression of Gelsolin in Colon Cancer and Its Diagnostic Value in Colon Cancer Patients
    Authors: Z Chen, K Li, X Yin, H Li, Y Li, Q Zhang, H Wang, Y Qiu
    J Cancer, 2019;10(5):1288-1296.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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