Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antibody

(7 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects endogenous human, mouse, and rat GPX1. In Western blots, this antibody shows no cross-reactivity with recombinant human GPX2 or GPX3.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Glutathione Peroxidase 1
    Gly48-Ala201
    Accession # P07203
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry

Glutathione Peroxidase 1/GPX1 in Rat Liver. Glutathione Peroxidase 1/GPX1 was detected in perfusion fixed frozen sections of rat liver using 15 µg/mL Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3798) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP‑DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Human/Mouse/Rat Glutathione Peroxidase 1 by Western Blot. Western blot shows lysates of human, mouse and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3798) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Glutathione Peroxidase 1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human and Mouse Glutathione Peroxidase 1/GPX1 by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Glutathione Peroxidase 1/GPX1 at approximately 30 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3798) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glutathione Peroxidase 1/GPX1

Glutathione Peroxidase 1 (GPX1), also known as Cellular Glutathione Peroxidase, is a 201 amino acid, 23 kDa member of the glutathione peroxidase antioxidant enzyme family. The Glutathione Peroxidase family protects cell surfaces, extracellular fluid components, and enzymes from oxidative stress by catalysing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX1 is a 92 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. GPX1 is an important antioxidant enzyme and protects red blood cells and other cells from oxidative damage. Deficiencies in red cell GPX1 have been associated with hemolytic anemia. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

  • Entrez Gene IDs:
    2876 (Human); 14775 (Mouse); 24404 (Rat)
  • Alternate Names:
    Cellular glutathione peroxidase; CGPx; EC 1.11.1; EC 1.11.1.9; Glutathione Peroxidase 1; GPX1; GPx-1; GSHPX1; GSHPx-1; MGC14399; MGC88245
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Global survey of cell death mechanisms reveals metabolic regulation of ferroptosis
    Nat Chem Biol, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Mice lacking selenoprotein P and selenocysteine lyase exhibit severe neurological dysfunction, neurodegeneration, and audiogenic seizures.
    Authors: Byrns, China N, Pitts, Matthew, Gilman, Christy, Hashimoto, Ann C, Berry, Marla J
    J Biol Chem, 2014;289(14):9662-74.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Isoform-specific binding of selenoprotein P to the beta-propeller domain of apolipoprotein E receptor 2 mediates selenium supply.
    Authors: Kurokawa S, Bellinger F, Hill K, Burk R, Berry M
    J Biol Chem, 2014;289(13):9195-207.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Evaluation of selenite effects on selenoproteins and cytokinome in human hepatoma cell lines.
    Authors: Rusolo, Fabiola, Pucci, Biagio, Colonna, Giovanni, Capone, Francesc, Guerriero, Eliana, Milone, Maria Ri, Nazzaro, Melissa, Volpe, Maria Gr, Di Bernardo, Gianni, Castello, Giuseppe, Costantini, Susan
    Molecules, 2013;18(3):2549-62.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Disruption of the selenocysteine lyase-mediated selenium recycling pathway leads to metabolic syndrome in mice.
    Authors: Seale L, Hashimoto A, Kurokawa S, Gilman C, Seyedali A, Bellinger F, Raman A, Berry M
    Mol Cell Biol, 2012;32(20):4141-54.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  6. Prostaglandin H Synthase-2-catalyzed Oxygenation of 2-Arachidonoylglycerol Is More Sensitive to Peroxide Tone than Oxygenation of Arachidonic Acid.
    Authors: Musee J, Marnett L
    J Biol Chem, 2012;287(44):37383-94.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  7. Resveratrol reduces endothelial oxidative stress by modulating the gene expression of superoxide dismutase 1 (SOD1), glutathione peroxidase 1 (GPx1) and NADPH oxidase subunit (Nox4).
    Authors: Spanier G, Xu H, Xia N
    J. Physiol. Pharmacol., 2009;60(0):111-6.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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