Human/Mouse/Rat Glutathione Peroxidase 4/GPX4 Antibody

Catalog #: MAB5457
11 Citations Datasheet / COA / SDS

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MAB5457
MAB5457-SP

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Detection of Human/Mouse/Rat Glutathione Peroxidase 4 by Western Blot.

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Citations (11)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Glutathione Peroxidase 4/GPX4 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat GPX4 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 565320

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Glutathione Peroxidase 4
Gly74-Phe168
Accession # P36969

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat Glutathione Peroxidase 4 by Western Blot. Western blot shows lysates of mouse, human, and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Glutathione Peroxidase 4 Monoclonal Antibody (Catalog # MAB5457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glutathione Peroxidase 4 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutathione Peroxidase 4/GPX4

Glutathione Peroxidase 4 (GPX4; also known as PHGPx) is a monomeric, 21 kDa member of the glutathione peroxidase family of proteins. It is widely expressed and serves to both protect cell membranes from phospholipid and cholesterol hydroperoxidases, and form an inactive enzyme structural component of the sperm mitochondrial capsule. Human GPX4 is 197 amino acids (aa) in length and contains a mitochondrial targeting sequence (aa 1‑27) and an enzymatically active selenocysteine at Sec73. During sperm maturation, the targeting sequence is cleaved, and GPX4 may form inactive covalently-linked oligomers. There are multiple splice variants. One is ubiquitously expressed, cytoplasmic, and shows an alternate start site at Met28. A second is 226 aa in length, nuclear, and shows an Arg-rich 65 aa substitution for aa 1‑28. Over amino acids 74‑168, human GPX4 shares 93% aa identity with mouse GPX4.

Entrez Gene IDs

2879 (Human); 625249 (Mouse); 29328 (Rat)

Alternate Names

EC 1.11.1; EC 1.11.1.12; glutathione peroxidase 4 (phospholipid hydroperoxidase); Glutathione Peroxidase 4; GPX4; GPx-4; GSHPx-4; MCSP; PHGPx; PHGPxsnGPx; phospholipid hydroperoxidase; phospholipid hydroperoxide glutathione peroxidase, mitochondrial; snGPx; snPHGPx; sperm nucleus glutathione peroxidase

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat Glutathione Peroxidase 4/GPX4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Acute lymphoblastic leukemia necessitates GSH-dependent ferroptosis defenses to overcome FSP1-epigenetic silencing
Authors: LB Pontel, A Bueno-Cost, AE Morellato, J Carvalho S, G Roué, M Esteller
Redox Biology, 2022;55(0):102408.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
A targetable CoQ-FSP1 axis drives ferroptosis- and radiation-resistance in KEAP1 inactive lung cancers
Authors: P Koppula, G Lei, Y Zhang, Y Yan, C Mao, L Kondiparth, J Shi, X Liu, A Horbath, M Das, W Li, MV Poyurovsky, K Olszewski, B Gan
Nature Communications, 2022;13(1):2206.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Upregulation of Nrf2 Signalling and the Inhibition of Erastin-Induced Ferroptosis by Ferulic Acid in MIN6 Cells
Authors: T Kose, PA Sharp, GO Latunde-Da
International Journal of Molecular Sciences, 2022;23(24):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Circulating hemopexin modulates anthracycline cardiac toxicity in patients and in mice
Authors: J Liu, S Lane, R Lall, M Russo, L Farrell, M Debreli Co, C Curtin, R Araujo-Gut, M Scherrer-C, BH Trachtenbe, J Kim, E Tolosano, A Ghigo, RE Gerszten, A Asnani
Science Advances, 2022;8(51):eadc9245.
Species: Mouse
Sample Types: Tissue Lysate
Applications: Western Blot
mTORC1 couples cyst(e)ine availability with GPX4 protein synthesis and ferroptosis regulation
Authors: Y Zhang, RV Swanda, L Nie, X Liu, C Wang, H Lee, G Lei, C Mao, P Koppula, W Cheng, J Zhang, Z Xiao, L Zhuang, B Fang, J Chen, SB Qian, B Gan
Nature Communications, 2021;12(1):1589.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
DHODH-mediated ferroptosis defence is a targetable vulnerability in cancer
Authors: C Mao, X Liu, Y Zhang, G Lei, Y Yan, H Lee, P Koppula, S Wu, L Zhuang, B Fang, MV Poyurovsky, K Olszewski, B Gan
Nature, 2021;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
A targetable LIFR-NF-kappaB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis
Authors: F Yao, Y Deng, Y Zhao, Y Mei, Y Zhang, X Liu, C Martinez, X Su, RR Rosato, H Teng, Q Hang, S Yap, D Chen, Y Wang, MM Chen, M Zhang, H Liang, D Xie, X Chen, H Zhu, JC Chang, MJ You, Y Sun, B Gan, L Ma
Nature Communications, 2021;12(1):7333.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Energy-stress-mediated AMPK activation inhibits ferroptosis
Authors: H Lee, F Zandkarimi, Y Zhang, JK Meena, J Kim, L Zhuang, S Tyagi, L Ma, TF Westbrook, GR Steinberg, D Nakada, BR Stockwell, B Gan
Nat. Cell Biol., 2020;22(2):225-234.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Epigenetic regulation of AURKA by miR-4715-3p in upper gastrointestinal cancers
Authors: A Gomaa, D Peng, Z Chen, M Soutto, K Abouelezz, A Corvalan, W El-Rifai
Sci Rep, 2019;9(1):16970.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Targeting redox homeostasis in rhabdomyosarcoma cells: GSH-depleting agents enhance auranofin-induced cell death
Authors: KJ Habermann, L Grünewald, S van Wijk, S Fulda
Cell Death Dis, 2017;8(10):e3067.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Impairment of antioxidant defense via glutathione depletion sensitizes acute lymphoblastic leukemia cells for Smac mimetic-induced cell death.
Authors: Schoeneberger H, Belz K, Schenk B, Fulda S
Oncogene, 2015;34(31):4032-43.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot