Human/Mouse/Rat HOMER1 Antibody

Catalog # Availability Size / Price Qty
MAB6889
MAB6889-SP
Detection of Human HOMER1 by Western Blot.
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Human/Mouse/Rat HOMER1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human HOMER1 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 666241
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Synthetic peptide KFGQWADSRA corresponding to aa 73-82 of human HOMER1
Accession # Q86YM7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
20 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human HOMER1 by Western Blot. View Larger

Detection of Human HOMER1 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue and IMR‑32 human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-HOMER1 Monoclonal Antibody (Catalog # MAB6889) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HOMER1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Simple Western Detection of Human and Mouse HOMER1 by Simple WesternTM. View Larger

Detection of Human and Mouse HOMER1 by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for HOMER1 at approximately 51 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat HOMER1 Monoclonal Antibody (Catalog # MAB6889). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HOMER1

HOMER1 is an approximately 48 kDa cytoplasmic protein that regulates the cell surface localization of neuronal mGluR1 and mGluR5 metabotropic glutamate receptors. It also functions in striated muscle mechanotransduction and excitation-contraction coupling by regulating the localization of transient receptor potential (TRP) channels and the coupling of ryanodine receptors to Cav1.2 calcium channels. Alternately spliced isoforms of human HOMER1 lack either the C-terminal half of the molecule (aa 178-354) or 130 aa following the WH1 domain (aa 99-228). Particular isoforms show distinct effects on hippocampal neuronal plasticity and the maintenance of extracellular glutamate levels in the prefrontal cortex. Within aa 73-82, human HOMER1 shares 100% aa sequence identity with mouse and rat HOMER1.

Long Name
Homer Protein Homolog 1
Entrez Gene IDs
9456 (Human); 26556 (Mouse)
Alternate Names
homer homolog 1 (Drosophila); HOMER; HOMER1; homer-1; HOMER1A; HOMER1B; HOMER-1B; HOMER1C; SYN47; SYN47neuronal immediate early gene, 1; Ves-1

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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