Detection of Human/Mouse/Rat HSP90 beta Isoform by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line, CH-1 mouse B cell lymphoma cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.25 µg/mL Mouse Anti-Human/Mouse/Rat HSP90 beta Monoclonal Antibody (Catalog # MAB32861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human HSP90 alpha and HSP90 beta (5 ng/lane) were included. A specific band for HSP90 beta was detected at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HSP90 beta
HSP90 is a composite name for a large group of Heat Shock Proteins whose molecular weights average 90 kDa. HSPs function primarily as a molecular chaperone, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. HSP90 is a central component in a number of basic cellular processes including hormone signaling and cell cycle control. HSP90 is ubiquitously expressed, highly conserved and accounts for 1-2% of the total cellular protein. Recently introduced, standardized nomenclature has divided the 17 identified HSP90 genes into three related and one unrelated classes, HSP90AA, HSP90AB, HSP90BB, and TRAP, respectively. Eukaryotic cells have two principal isoforms of HSP90. The antibody described here reacts with the constitutively expressed, 724 amino acid, HSP90AB1-isoform 1 (also known as HSP90 beta, HSP90B, HSPCB, HSPC2, and HSP89 beta ). The inducible form, HSP90AA1, is a 737 amino acid protein (also known as HSP90 alpha, HSP90A, HSPC1, HSPCA, HSP89 alpha and LAP2). HSP90AB1-1 and HSP90AA share 90% identity.
Heat Shock Protein 90 beta
Entrez Gene IDs:
D6S182; Heat shock 84 kDa; heat shock 90kD protein 1, beta; heat shock 90kDa protein 1, beta; heat shock protein 90kDa alpha (cytosolic), class B member 1; heat shock protein beta; heat shock protein HSP 90-beta; HSP 84; HSP84; HSP90 beta; HSP90AB1; HSP90B; HSP90-BETA; HSP90BHSP 90; HSPC2; HSPC2D6S182; HSPCB; HSPCBFLJ26984
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