Human/Mouse/Rat ILK Antibody

Catalog # Availability Size / Price Qty
MAB4266
MAB4266-SP
Detection of Human/Mouse/Rat ILK by Western Blot.
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Reviews (5)

Human/Mouse/Rat ILK Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat ILK in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 443208
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ILK
Met1-Lys452
Accession # Q13418
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human/Mouse/Rat ILK antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat ILK by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and PT18 mouse mast/basophil cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat ILK Monoclonal Antibody (Catalog # MAB4266) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human ILK (1 ng) was included. A specific band for ILK was detected at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ILK

Integrin-linked kinase (ILK) is an ankyrin repeat-containing serine/threonine protein kinase that interacts with the cytoplasmic domain of beta 1 and beta 3 integrins. ILK functions with multiple binding partners to transmit signals from the extracellular matrix to actin filaments, regulating such processes as cellular attachment, cell cycle progression, and cytoskeletal organization. ILK kinase activity is induced by PI3 kinase and negatively regulated by the tumor suppressor PTEN.

Long Name
Integrin-linked Kinase
Entrez Gene IDs
3611 (Human); 16202 (Mouse); 170922 (Rat)
Alternate Names
59 kDa serine/threonine-protein kinase; DKFZp686F1765; EC 2.7.11.1; ESTM24; ILK; ILK1; ILK-1; ILK2; ILK-2; integrin-linked kinase; integrin-linked kinase-2; integrin-linked protein kinase; p59; p59ILK

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat ILK Antibody

Average Rating: 4.4 (Based on 5 Reviews)

5 Star
60%
4 Star
20%
3 Star
20%
2 Star
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1 Star
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Human/Mouse/Rat ILK Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse/Rat ILK Antibody
By Anonymous on 02/08/2020
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma


Human/Mouse/Rat ILK Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse/Rat ILK Antibody
By Anonymous on 06/21/2018
Application: ICC/IF Sample Tested: Trabecular meshwork Species: Human

Green: F-actin
Red: ILK
Blue: Nuclear


Human/Mouse/Rat ILK Antibody
By Anonymous on 01/25/2018
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human