Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Rabbit, Transgenic Mouse, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2D2-B2
View all iNOS Primary Antibodies »

Product Specifications

Immunogen

Recombinant human iNOS
Pro781-His798
Accession # P35228

Specificity

Detects human iNOS. By using synthetic peptides, the epitope recognized by this antibody has been mapped to aa 781-798 of human iNOS. The corresponding sequence of mouse iNOS is identical.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat iNOS Antibody

Detection of Human iNOS antibody by Western Blot.

Detection of Human iNOS by Western Blot.

Western blot shows lysates of DLD clone 2C2 human colon adenocarcinoma cell line untreated (-) or treated (+) with Recombinant Human IL-1 beta, Recombinant Human TNF-alpha, and Recombinant Human IFN-gamma (Catalog # HAF007). A specific band was detected for iNOS at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.

Detection of Mouse and Rat iNOS antibody by Western Blot.

Detection of Mouse and Rat iNOS by Western Blot.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for iNOS at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

iNOS antibody in Human Brain by Immunohistochemistry (IHC-P).

iNOS in Human Brain.

iNOS was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Mouse iNOS antibody by Simple WesternTM.

Detection of Mouse iNOS by Simple WesternTM.

Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS, loaded at 0.2 mg/mL. A specific band was detected for iNOS at approximately 136 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Rat iNOS by Western Blot

Detection of Rat iNOS by Western Blot

Ultrasound (US) stimulation reduces ED-1, iNOS, and TNF-alpha expression in the acute spinal cord injury (SCI) phase. (A–C) ED-1 (macrophage/microglia, green) and iNOS (inducible nitric oxide synthase, red) were evaluated in the sham (A), SCIU40 (B), and SCIU5 (c) experimental groups at 7 days post-SCI (five sections per rat, n = 3). (D–F) Cross-sections were counterstained with anti-TNF-alpha (red) and ED-1 (green) antibodies (five sections per rat, n = 3). (G–I) Quantification of fluorescence intensity of ED-1, iNOS, and TNF-alpha. Two-way ANOVA with Turkey's test for multiple comparisons was used for analyses. *P < 0.05, ****P < 0.0001 compared with the sham control, #P < 0.05, ###P < 0.001, ####P < 0.0001 compared with the SCIU5-treated group. (J) Representative western blotting results for ED-1, iNOS, TNF-alpha, and GAPDH at 7 days post-SCI (Lane 1, sham; lane 2, SCIU40; lane 3, SCIU5;). Relative expression levels of ED-1, iNOS, and TNF-alpha were calculated after normalization to the level of GAPDH. Full-length blots are presented in Supplementary Fig. 8. Data are presented as means ± SEM. Two-tailed Student’s t-tests were used for comparison. *P < 0.05 compared with the sham control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35256617), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat iNOS Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (medulla).

Simple Western

10 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line treated with LPS

Western Blot

1 µg/mL
Sample: DLD clone 2C2 human colon adenocarcinoma cell line treated with Recombinant Human IL-1 beta, Recombinant Human TNF-alpha, and Recombinant Human IFN-gamma (Catalog # 201-LB, 210-TA, 285-IF), RAW 264.7 mouse monocyte/macrophage cell line treated with LPS, and NR8383 rat alveolar macrophage cell line treated with LPS

Reviewed Applications

Read 9 reviews rated 3.9 using MAB9502 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: iNOS

Inducible nitric oxide synthase (iNOS) is a widely expressed, approximately 130 kDa enzyme that catalyzes the conversion of L-arginine to nitric oxide (NO) plus citrulline. NO is a bioactive mediator that plays an important role in hemodynamics by inducing vascular smooth muscle relaxation. iNOS is constitutively expressed in the kidney. In other tissues, it is inducibly expressed during inflammation, oxidative stress, and hyperglycemia. iNOS activity is elevated in a variety of disease states including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Increased production of NO mediates acute kidney injury, TNF-alpha induced muscle wasting, and the increased radiosensitivity of hypoxic tumor cells. Within amino acids 781-798, human iNOS shares 89% and 72% aa sequence identity with mouse and rat iNOS, respectively.

Long Name

Inducible Nitic Oxide Synthase

Alternate Names

NOS2, NOS2A

Entrez Gene IDs

4843 (Human); 18126 (Mouse)

Gene Symbol

NOS2

UniProt

P35228

Additional iNOS Products

Product Documents for Human/Mouse/Rat iNOS Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat iNOS Antibody

For research use only

Citations for Human/Mouse/Rat iNOS Antibody

Customer Reviews for Human/Mouse/Rat iNOS Antibody (9)

3.9 out of 5
9 Customer Ratings
5 Stars
33%
4 Stars
44%
3 Stars
11%
2 Stars
0%
1 Stars
11%

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Customer Images

Showing  1 - 5 of 9 reviews Showing All
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  • Human/Mouse/Rat iNOS Antibody
    Name: Edma Loku
    Application: Western Blot
    Sample Tested: Lung homogenates
    Species: Mouse
    Verified Customer | Posted 10/30/2025
    Didn't work well. Somehow I couldn't get the inos signal.
  • Human/Mouse/Rat iNOS Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Lung tissue
    Species: Human
    Verified Customer | Posted 04/11/2023
    Leica Bond ER2 heat retrieval primary Ab MAB9502 at 5ug/mL in BSA-TBS Leica standard IHC protocol on Leica Rx stainer
    Human/Mouse/Rat iNOS Antibody MAB9502
  • Human/Mouse/Rat iNOS Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Bone marrow tissue
    Species: Mouse
    Verified Customer | Posted 08/13/2021
    Human/Mouse/Rat iNOS Antibody MAB9502
  • Human/Mouse/Rat iNOS Antibody
    Name: Xue Yuan
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Connective tissue
    Species: Mouse
    Verified Customer | Posted 07/06/2021
    Human/Mouse/Rat iNOS Antibody MAB9502
  • Human/Mouse/Rat iNOS Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line
    Species: Human and Mouse
    Verified Customer | Posted 04/03/2021
    Antibody also works very well for immunohistochemistry
    Human/Mouse/Rat iNOS Antibody MAB9502
  • Human/Mouse/Rat iNOS Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Adult brain
    Species: Rat
    Verified Customer | Posted 07/28/2020
    Rats were perfused with 4% paraformaldehyde in PBS (pH=7.6). The brain was removed and immersed in PFA then in 30% sucrose. The brain was sliced with a freezing microtome. A mixture of 1% Triton-X and 10% donkey serum (in TRIS) was used for antigen retrieval and blocking. Tmem119 antibody was used in 1:100 dilution and was visualized by a Cy3 conjugated anti-mouse secondary antibody (1:500, yellow). Cell bodies were stained with NeuroTrace 640/660 (1:250, red). Pictures show a part of the rat hippocampus. The product was assumed to label activated microglial cells. It seems, it did not. However, it labelled some cerebral blood vessels, which is also meaningful, thus, it may be useful for this application.
    Human/Mouse/Rat iNOS Antibody MAB9502
  • Human/Mouse/Rat iNOS Antibody
    Name: Lenka Uherková
    Application: Simple Western
    Sample Tested: skin wound - diabetic
    Species: Rat
    Verified Customer | Posted 02/12/2020
    Human/Mouse/Rat iNOS Antibody MAB9502
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 21075192
    Species: Mouse
    Verified Customer | Posted 02/25/2015
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 23147558
    Species: Human
    Verified Customer | Posted 02/25/2015

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