Muscle Phosphofructokinase/PFKM Antibody (842735)

R&D Systems | Catalog # MAB7687

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 842735
View all Muscle Phosphofructokinase/PFKM Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Muscle Phosphofructokinase/PFKM
Asn674-Val780
Accession # P08237

Specificity

Detects human Muscle Phosphofructokinase/PFKM in direct ELISAs and Western blots. Detects mouse and rat Muscle Phosphofructokinase/PFKM in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Muscle Phosphofructokinase/PFKM Antibody (842735)

Detection of Human, Mouse, and Rat Muscle Phosphofructokinase/PFKM antibody by Western Blot.

Detection of Human, Mouse, and Rat Muscle Phosphofructokinase/PFKM by Western Blot.

Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Muscle Phospho-fructokinase/PFKM Monoclonal Antibody (Catalog # MAB7687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Muscle Phosphofructokinase/PFKM at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Rat Muscle Phosphofructokinase/PFKM antibody by Simple WesternTM.

Detection of Human and Rat Muscle Phosphofructokinase/PFKM by Simple WesternTM.

Simple Western lane view shows lysates of human heart tissue and rat heart tissue, loaded at 0.5 mg/mL. A specific band was detected for Muscle Phosphofructokinase/PFKM at approximately 84 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human/Mouse/Rat Muscle Phosphofructokinase/PFKM Monoclonal Antibody (Catalog # MAB7687). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Muscle Phosphofructokinase/PFKM Antibody (842735)

Application
Recommended Usage

Simple Western

2 µg/mL
Sample: Human heart tissue and rat heart tissue

Western Blot

0.2 µg/mL
Sample: Human heart tissue, mouse heart tissue, and rat heart tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Muscle Phosphofructokinase/PFKM

PFKM (PhosphoFructoKinase, Muscle type; also PFK-A and Phosphohexokinase)) is a 85-86 kDa member of the phosphofructokinase family, two domain subfamily of enzymes. It is one of three functionally related enzymes, all expressed by distinct genes. While PFKM is expressed by virtually all cells, its name is based on the fact that skeletal muscle expresses only the PFKM isotype. PFK catalyzes the essentially irreversible phosphorylation of Fru-6-P, forming Fru-1,6-bisP. This determines the rate of cellular glycolysis. As a monomer, PFKM is unstable, and following synthesis, PFKM forms low activity homodimers. High activity comes with tetramerization/oligomerization. In all cases, multimerization is associated with PFKM interaction with various components of the cell cytoskeleton. Human PFKM is 780 amino acids (aa) in length. It contains a 740 aa catalytic region (aa 16-755) plus one utilized phosphorylation site at Thr2. There are at least two potential isoform variants. One contains an alternative start site 71 aa upstream of the standard site, while another shows a deletion of aa 282-312. Proteolysis of PKFM apparently occurs, generating 45-47 kDa fragments. Over aa 674-780, human PFKM shares 98% aa sequence identity with mouse PFKM.

Long Name

Phosphofructokinase, Muscle

Alternate Names

GSD7, PFK-1, PFK-A, PFKM, PFKX, Phosphofructokinase-1, Phosphofructokinase-M, Phosphohexokinase

Entrez Gene IDs

5213 (Human); 18642 (Mouse); 65152 (Rat)

Gene Symbol

PFKM

UniProt

P08237 (Human)

Additional Muscle Phosphofructokinase/PFKM Products

Product Documents for Muscle Phosphofructokinase/PFKM Antibody (842735)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Muscle Phosphofructokinase/PFKM Antibody (842735)

For research use only

Related Research Areas

Glycobiology-related Enzymes

Citations for Muscle Phosphofructokinase/PFKM Antibody (842735)

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