NLRP10/Pynod/NALP10 Antibody (578502)

Detection of Human, Mouse, and Rat NLRP10/Pynod/NALP10 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HT-2 mouse T cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Human/Mouse/Rat NLRP10/Pynod/NALP10 Monoclonal Antibody (Catalog # MAB6606) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NLRP10/Pynod/ NALP10 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of e2ciit7p91nfjzkmnpso.jpg by Western Blot

NLRP10 binds non-oxidized mitochondrial DNA.A Superposition of NLRP10 (gray PDB 2M5V) with hOGG1 (rainbow PDB 8VX6) interrogating a non-oxidized guanine. B Superposition from (A) with residues within 5 Å from non-oxidized guanine shown in magenta and lysine close to the catalytic site shown in cyan. C SDS Page gel following flag column purification of NLRP10 (left). PVDF membrane probed for NLRP10 following FLAG column purification (right). D Fluorescence polarization of cy5-labeled 20 bp non-oxidized DNA incubated with increasing concentrations of NLRP10. Error bars: mean ± SEM, analyzed with one-way ANOVA. N = 3, ∗∗p = 0.0011 and 0.0072 and ∗∗∗p = 0.0003, 0.0009, and 0.0007. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s42003-025-09501-x), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of NLRP10/Pynod/NALP10 by Western Blot

NLRP10 directly binds NLRP3, and the association decreases upon activation.A IL-1 beta western blots of LPS-primed and nigericin-activated THP-1 cells treated with increasing doses of TH5487. Ponceau S. stain serves as a loading control. Representative pro-IL-1 beta western blot. Quantification of IL-1 beta western blot data. Error bars: mean ± SEM, analyzed with one-way ANOVA. N = 3, ∗∗∗∗p < 0.0001, ∗∗∗p = 0.0003, 0.0008, ∗p = 0.0154. B IL-1 beta western blots of LPS-primed and nigericin-activated THP-1 cells treated with increasing doses of SU0268. Ponceau S. stain serves as a loading control. Representative pro-IL-1 beta western blot. Quantification of IL-1 beta western blot data. Error bars: mean ± SEM, analyzed with one-way ANOVA. N = 3, ∗∗p = 0.0026, ∗p = 0.0174. C NLRP3 IP pulldown of NLRP10 in LPS-primed nigericin-activated THP-1 cells. D Quantification of Western blot data. Error bars: mean ± SEM, analyzed with one-way ANOVA. N = 3, ∗∗∗∗p < 0.0001. E Coomassie SDS gels following affinity chromatography for NLRP10 (left) and size exclusion chromatography for NLRP3 (right). F NLRP3 IP pulldown of NLRP10 with purified protein. G Quantification of Western blot data. Mean ± SEM, one-way ANOVA. N = 3, ∗p = 0.0129, ∗∗∗p = 0.0007. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s42003-025-09501-x), licensed under a CC-BY license. Not internally tested by R&D Systems.