Human/Mouse/Rat NLRP10/Pynod/NALP10 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human NLRP10/Pynod/NALP10 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) NALP1 (aa 1‑323), rhNALP1 (aa 1331‑1429), rhNALP2 (aa 1‑208), rhNALP3 (aa 3‑223), rhNALP3 (aa 540‑689), rhNALP5 (aa 469‑635), rhNOD1 (aa 830‑953), or rhNOD2 (aa 230‑450) is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 578502
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human NLRP10/Pynod/NALP10
    Met386-Ile655
    Accession # Q86W26
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat NLRP10/Pynod/NALP10 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HT‑2 mouse T cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Human/Mouse/Rat NLRP10/Pynod/NALP10 Monoclonal Antibody (Catalog # MAB6606) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NLRP10/Pynod/
NALP10 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NLRP10/Pynod/NALP10

NLRP10, also known as Pynod, NALP10, and NOD8, is a 75 kDa cytoplasmic protein that associates with the ASC component of inflammasomes. NLRP10 inhibits ASC‑dependent apoptosis, NFkB activation, and Caspase-1-dependent processing of IL-1 beta. Human NLRP10 contains one Pyrin domain (aa 1-96) and one NACHT domain (aa 167-484) but no leucine-rich repeats. Within aa 386-655, human NLRP10 shares approximately 50% aa sequence identity with mouse and rat NLRP10.

  • Long Name:
    NLR Family, Pyrin Domain Containing 10
  • Entrez Gene IDs:
    338322 (Human); 244202 (Mouse); 293426 (Rat)
  • Alternate Names:
    CLR11.1; NACHT, leucine rich repeat and PYD containing 10; NALP10; NALP10Pynod; NLR family, pyrin domain containing 10; NLRP10; NOD8; NOD8Nucleotide-binding oligomerization domain protein 8; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 10; PAN5; Pynod; PYNODNACHT, LRR and PYD domains-containing protein 10
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Mouse IgG2B Isotype Control

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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Mouse IgG Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

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Mouse IgG2B Antibody

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Rat Anti-Mouse IgG2B PE-conjugated Antibody

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