Detects human NLRP10/Pynod/NALP10 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) NALP1 (aa 1‑323), rhNALP1 (aa 1331‑1429), rhNALP2 (aa 1‑208), rhNALP3 (aa 3‑223), rhNALP3 (aa 540‑689), rhNALP5 (aa 469‑635), rhNOD1 (aa 830‑953), or rhNOD2 (aa 230‑450) is observed.
Monoclonal Mouse IgG2B Clone # 578502
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human NLRP10/Pynod/NALP10 Met386-Ile655 Accession # Q86W26
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat NLRP10/Pynod/NALP10 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HT‑2 mouse T cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Human/Mouse/Rat NLRP10/Pynod/NALP10 Monoclonal Antibody (Catalog # MAB6606) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NLRP10/Pynod/ NALP10 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
NLRP10, also known as Pynod, NALP10, and NOD8, is a 75 kDa cytoplasmic protein that associates with the ASC component of inflammasomes. NLRP10 inhibits ASC‑dependent apoptosis, NFkB activation, and Caspase-1-dependent processing of IL-1 beta. Human NLRP10 contains one Pyrin domain (aa 1-96) and one NACHT domain (aa 167-484) but no leucine-rich repeats. Within aa 386-655, human NLRP10 shares approximately 50% aa sequence identity with mouse and rat NLRP10.
NLR Family, Pyrin Domain Containing 10
Entrez Gene IDs:
338322 (Human); 244202 (Mouse); 293426 (Rat)
CLR11.1; NACHT, leucine rich repeat and PYD containing 10; NALP10; NALP10Pynod; NLR family, pyrin domain containing 10; NOD8; NOD8Nucleotide-binding oligomerization domain protein 8; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 10; PAN5; Pynod; PYNODNACHT, LRR and PYD domains-containing protein 10
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.