Human/Mouse/Rat NM23-H1/H2 Antibody

Catalog # Availability Size / Price Qty
MAB6019
MAB6019-SP
Detection of Human, Mouse, and Rat NM23‑H1/H2 by Western Blot.
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Human/Mouse/Rat NM23-H1/H2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat NM23‑H1/H2 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 642825
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human NM23‑H1/H2
Ala2-Glu152
Accession # P15531
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat NM23-H1/H2 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat NM23‑H1/H2 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, HT-2 mouse T cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat NM23-H1/H2 Monoclonal Antibody (Catalog # MAB6019) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human NM23-H1 and recombinant human NM23-H2 (10 ng/lane) were included. Specific bands were detected for NM23-H1/H2 at approximately 17-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NM23-H1/H2

NM23-H1 (Non-metastatic protein 23 homolog 1; also NDKA and NME1) is a 19‑20 kDa member of the NDK family of enzymes. NM23-H1 is ubiquitous in expression, and performs multiple functions. It forms disulfide-linked homohexamers, and heterohexamers with NM23-H2, generating a nucleoside diphosphate kinase that catalyzes a phosphoryl transfer from ATP to a nucleoside diphosphate. It also shows His and Ser/Thr protein kinase activity, and forms covalent linkages with molecules diverse as p53 and STRAP. It is found both intracellularly, and in blood at ng/mL concentrations. Human NM23-H1 is 152 amino acids (aa) in length, contains one NDP kinase domain (aa 5‑134) and shows acetylation at Ala2 and Lys56, plus phosphorylation at Tyr52, Thr94, Ser122 and Ser125. Human NM23-H1 shares 89% aa identity with human 17‑18 kDa NM23-H2, and 94% aa identity with mouse NM23-H1. A second H1 isoform named NM23-H1B with 25 additional aa at the N‑terminus has also been described.

Long Name
Non-metastatic Protein 23 Homolog 1/Homolog 2
Entrez Gene IDs
4830 (Human)
Alternate Names
AL024257;AWD;GAAD;NB;NBS;NDKA;NDKB;NDPKA;NDPK-A;NDPKB;NDPK-B;NM23;nm23-2;NM23A;NM23B;NM23-H1;NM23-H2;NM23-M1;nm23-M2;NME1;NME2;p18-12d;PUF; NM23-H1/H2; NM23H2

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