Human/Mouse/Rat p27/Kip1 Antibody Summary
Accession # P46527
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Mouse/Rat p27/Kip1 by Western Blot. Western blot shows lysates of NRK rat normal kidney cell line, U2OS human osteosarcoma cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat p27/Kip1 Monoclonal Antibody (Catalog # MAB2256) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p27/Kip1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27/Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle.
Citations for Human/Mouse/Rat p27/Kip1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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NK-cell dysfunction in human renal carcinoma reveals diacylglycerol kinase as key regulator and target for therapeutic intervention.
Authors: Prinz P, Mendler A, Brech D, Masouris I, Oberneder R, Noessner E
Int J Cancer, 2014;135(8):1832-41.
Sample Types: Whole Cells
Applications: Flow Cytometry
FOXO3a is involved in the apoptosis of naked oocytes and oocytes of primordial follicles from neonatal rat ovaries.
Authors: Liu H, Luo LL, Qian YS, Fu YC, Sui XX, Geng YJ, Huang DN, Gao ST, Zhang RL
Biochem. Biophys. Res. Commun., 2009;381(4):722-7.
Sample Types: Cell Lysates
Applications: Western Blot
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