Human/Mouse/Rat p38 gamma Antibody

Name: Human/Mouse/Rat p38 gamma Antibody
Brand: R&D Systems
SKU: AF1644
Price: 449 USD
Availability: InStock

R&D Systems | Catalog # AF1644

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all p38 gamma Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human p38 gamma
Met1-Leu367
Accession # P53778

Specificity

Detects human, mouse, and rat p38 gamma in Western blots. The antibody is highly specific for p38 gamma and in Western blots, shows extremely weak cross-reactivity with recombinant p38 alpha, p38 beta, or p38 delta after prolonged exposure to film.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat p38 gamma Antibody

Detection of Human, Mouse, and Rat p38 gamma by Western Blot.

Western blot shows lysates of C2C12 mouse myoblast cell line myoblast cell line and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.2 µg/mL Goat Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1644) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human p38 beta, p38 gamma, p38d and p38a (5 ng/lane) were included. A specific band for p38 gamma was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat p38 gamma Antibody

Application

Recommended Usage

Western Blot

0.2 µg/mL
Sample: C2C12 mouse myoblast cell line myoblast cell line and PC-12 rat adrenal pheochromocytoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: p38 gamma

p38 mitogen-activated protein kinase (MAPK)gamma, also known as p38 gamma, MAPK12, SAPK3 and ERK6 is a serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells.