Detection of Human, Mouse, and Rat PDCD5 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line, EL‑4 mouse lymphoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat PDCD5 Polyclonal Antibody (Catalog # MAB7325) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PDCD5 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PDCD5 in Human Stomach.
PDCD5 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human/Mouse/Rat PDCD5 Monoclonal Antibody (Catalog # MAB7325) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
PDCD5 (programmed cell death 5), also called TFAR19 (TF-1 cell apoptosis-related 19) is a ubiquitously expressed, approximately 15 kDa protein that is upregulated during apoptosis and downregulated in many cancer cell types. Of its 124 amino acids (aa), 44 are acidic or basic. PDCD5 also contains two acetylated aa and one constitutively phosphorylated aa, S119, which is involved in promoting apoptosis. Human PDCD5 shares 95% and 96% aa sequence identity with mouse and rat PDCD5, respectively.
Programmed Cell Death 5
Entrez Gene IDs:
9141 (Human); 56330 (Mouse); 292814 (Rat)
FLJ42784; MGC9294; PD5; PDCD5; programmed cell death 5; programmed cell death protein 5; Protein TFAR19; TF-1 cell apoptosis-related protein 19; TFAR19 novel apoptosis-related; TFAR19; TFAR19TF1 cell apoptosis-related gene 19
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