Detection of Human, Mouse, and Rat PDCD6 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Hepa 1‑6 mouse hepatoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat PDCD6 Monoclonal Antibody (Catalog # MAB6015) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PDCD6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
PDCD6 (Programmed cell death protein 6; also ALG-2 and apoptosis-linked gene 2 protein) is a 22 kDa member of the penta-EF hand protein family of molecules. It is ubiquitously expressed, and performs multiple functions. PDCD6 is contextually linked to apoptosis, proliferation, membrane fusion and vesicle trafficking. Human PDCD6 is 191 amino acids (aa) in length. Although it contains five EF-hand domains, only domains #1 and #3 are considered functional (aa 23-58 and 90‑125). There is one splice form (ALG-2.1) that shows a deletion of Gly121Phe122. This form typically represents about 1/3 of total cellular PDCD6. PDCD6/ALG-2 will homodimerize, and heterodimerize with ALG-2.1. Notably, there are classes of cytosolic proteins that bind PDCD6, but not ALG-2.1, accounting for differences in function. Both human PDCD6 isoforms share 99% aa identity with their mouse counterparts.
Programmed Cell Death 6
Entrez Gene IDs:
ALG2; ALG-2; Apoptosis-linked gene 2 protein; apoptosis-linked gene-2; FLJ46208; MGC111017; MGC119050; MGC9123; PDCD6; PEF1B; Probable calcium-binding protein ALG-2; programmed cell death 6; programmed cell death protein 6
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