Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Peroxiredoxin 4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Peroxiredoxin 4
Met1-Asn271
Accession # Q13162

Specificity

Detects human, mouse and rat Peroxiredoxin 4 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Peroxiredoxin 4 Antibody

Detection of Human/Mouse/Rat Peroxiredoxin 4 antibody by Western Blot.

Detection of Human/Mouse/Rat Peroxiredoxin 4 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, K562 human chronic myelogenous leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5460) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Peroxiredoxin 4 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Peroxiredoxin 4 antibody in Human Liver Cancer Tissue by Immunohistochemistry (IHC-P).

Peroxiredoxin 4 in Human Liver Cancer Tissue.

Peroxiredoxin 4 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human/Mouse/Rat Peroxiredoxin 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5460) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Mouse/Rat Peroxiredoxin 4 Antibody

Application
Recommended Usage

Immunohistochemistry

0.3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver cancer tissue

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, K562 human chronic myelogenous leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cells

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Peroxiredoxin 4

Peroxiredoxin 4 (Prx-IV; also known as PRDX4 and thioredoxin peroxidase AOE372) is a 30-32 kDa, widely expressed cytoplasmic antioxidant enzyme that belongs to the typical 2-Cys class of the TSA/ahpC family of peroxiredoxins. It exists as either a homodimer, or heterodimer with PRDX1. PRDX4 reportedly counteracts
p53‑mediated generation of ROS, and regulates NF kappa B via I kappa B-alpha. Human PRDX4 is 271 amino acids (aa) in length and contains an N-terminal poly-Leu region
(aa 20‑30) and a 159 thioredoxin domain (aa 79‑237). There is one catalytic cysteine at Cys124. Over amino acids 1‑271, human PRDX4 shares 89% aa identity with mouse and rat PRDX4.

Alternate Names

AOE372, PRDX4, Prx4

Entrez Gene IDs

10549 (Human); 53381 (Mouse); 85274 (Rat)

Gene Symbol

PRDX4

UniProt

Q13162 (Human)

Additional Peroxiredoxin 4 Products

Product Documents for Human/Mouse/Rat Peroxiredoxin 4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat Peroxiredoxin 4 Antibody

For research use only

Related Research Areas

Oxidative Stress
Redox Enzymes

Citations for Human/Mouse/Rat Peroxiredoxin 4 Antibody

Customer Reviews for Human/Mouse/Rat Peroxiredoxin 4 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat Peroxiredoxin 4 Antibody and earn rewards!

Have you used Human/Mouse/Rat Peroxiredoxin 4 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies