Human/Mouse/Rat Peroxiredoxin 4 Antibody

Catalog # Availability Size / Price Qty
AF5460
AF5460-SP
Human/Mouse/Rat Peroxiredoxin 4 Antibody in Western Blot
2 Images
Product Details
Citations (1)
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Human/Mouse/Rat Peroxiredoxin 4 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat Peroxiredoxin 4 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Peroxiredoxin 4
Met1-Asn271
Accession # Q13162
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
0.3-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human/Mouse/Rat Peroxiredoxin 4 by Western Blot. View Larger

Detection of Human/Mouse/Rat Peroxiredoxin 4 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, K562 human chronic myelogenous leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5460) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Peroxiredoxin 4 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunohistochemistry Peroxiredoxin 4 in Human Liver Cancer Tissue. View Larger

Peroxiredoxin 4 in Human Liver Cancer Tissue. Peroxiredoxin 4 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human/Mouse/Rat Peroxiredoxin 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5460) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Peroxiredoxin 4

Peroxiredoxin 4 (Prx-IV; also known as PRDX4 and thioredoxin peroxidase AOE372) is a 30-32 kDa, widely expressed cytoplasmic antioxidant enzyme that belongs to the typical 2-Cys class of the TSA/ahpC family of peroxiredoxins. It exists as either a homodimer, or heterodimer with PRDX1. PRDX4 reportedly counteracts
p53‑mediated generation of ROS, and regulates NF kappa B via I kappa B-alpha. Human PRDX4 is 271 amino acids (aa) in length and contains an N-terminal poly-Leu region
(aa 20‑30) and a 159 thioredoxin domain (aa 79‑237). There is one catalytic cysteine at Cys124. Over amino acids 1‑271, human PRDX4 shares 89% aa identity with mouse and rat PRDX4.

Entrez Gene IDs
10549 (Human); 53381 (Mouse); 85274 (Rat)
Alternate Names
Antioxidant enzyme AOE372; AOE372; AOE37-2PRX-4; EC 1.11.1.15; Peroxiredoxin 4; Peroxiredoxin IV; peroxiredoxin-4; PRDX4; Prx4; prx-4; prx-IV; thioredoxin peroxidase (antioxidant enzyme); Thioredoxin peroxidase AO372; Thioredoxin-dependent peroxide reductase A0372

Product Datasheets

Citation for Human/Mouse/Rat Peroxiredoxin 4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. 1,25-Dihydroxy vitamin D prevents tumorigenesis by inhibiting oxidative stress and inducing tumor cellular senescence in mice
    Authors: L Chen, R Yang, W Qiao, X Yuan, S Wang, D Goltzman, D Miao
    Int. J. Cancer, 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Secondary Antibodies

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