Home / Akt / Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antibody

Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antibody

Catalog #: AF887
30 Citations Datasheet / COA / SDS
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AF887
AF887-SP

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Detection of Mouse and Human Phospho-Akt (S473) Pan Specific by Western Blot.
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Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat Akt1, Akt2 and Akt3, when phosphorylated at S473, S474 and S472, respectively.
Source
Polyclonal Rabbit IgG
Purification
Antigen and protein A Affinity-purified
Immunogen
Phosphopeptide containing human, mouse, and rat Akt1 S473 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Human Phospho-Akt (S473) Pan Specific antibody by Western Blot. View Larger

Detection of Mouse and Human Phospho-Akt (S473) Pan Specific by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120-HD) for 15 minutes and MCF-7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291-G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Akt antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Akt in Human Breast Cancer Tissue. Akt phosphorylated at S473 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Intracellular Staining by Flow Cytometry Detection of Phospho-Akt (S473) antibody in IGF-1-treated MCF-7 cells antibody by Flow Cytometry. View Larger

Detection of Phospho-Akt (S473) in IGF-1-treated MCF‑7 cells by Flow Cytometry. Serum-starved MCF-7 human breast cancer cells were unstimulated (light orange filled histogram) or activated with 100 ng/mL Recombinant human IGF-1 (Catalog # 291-G1, dark orange filled histogram) for 15 minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Simple Western Detection of Human Phospho-Akt (S473) antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Phospho-Akt (S473) by Simple WesternTM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Akt

Akt1, Akt2, and Akt3 make up a family of serine/threonine kinases, often activated downstream of growth factor receptors and PI 3-Kinase. Each Akt family member contains an N-terminal pleckstrin homology (PH) domain, a central kinase domain, and a C-terminal regulatory domain. They have putative roles in cell proliferation and survival, and Akt perturbations are thought to play an important role in tumorigenesis.

Long Name
v-Akt Murine Thymoma Viral Oncogene Homolog
Alternate Names
RAC-ALPHA; AKT serine/threonine kinase 1; Akt; PKB; PKB-ALPHA; PRKBA; RAC

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Citations for Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. Morin post-treatment surpassed calpeptin in ameliorating 3-NP-induced cortical neurotoxicity via modulation of glutamate/calpain axis, Kidins220, and BDNF/TrkB/AKT/CREB trajectory
    Authors: OE Mohamed, DM Abdallah, AM Fayez, RA Mohamed, HS El-Abhar
    International immunopharmacology, 2023-02-01;116(0):109771.
    Species: Rat
    Sample Types: Protein
    Applications: Western Blot
  2. Dock7 regulates AKT and mTOR/S6K activity required for the transformed phenotypes and survival of cancer cells
    Authors: OY Teran, MR Zanotelli, MJ Lin, RA Cerione, KF Wilson
    bioRxiv : the preprint server for biology, 2023-01-03;0(0):.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. Effects of American wild ginseng and Korean cultivated wild ginseng pharmacopuncture extracts on the regulation of C2C12 myoblasts differentiation through AMPK and PI3K/Akt/mTOR signaling pathway
    Authors: JH Hwang, SY Kang, HW Jung
    Molecular Medicine Reports, 2022-04-08;25(6):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Nickel's Role in Pancreatic Ductal Adenocarcinoma: Potential Involvement of microRNAs
    Authors: M Mortoglou, L Mani?, A Buha Djord, Z Bulat, V ?or?evi?, K Manis, E Valle, L York, D Wallace, P Uysal-Onga
    Toxics, 2022-03-21;10(3):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Development
  5. Multiplex coherent anti-Stokes Raman scattering microspectroscopy detection of lipid droplets in cancer cells expressing TrkB
    Authors: T Guerenne-D, V Couderc, L Duponchel, V Sol, P Leproux, JM Petit
    Sci Rep, 2020-10-07;10(1):16749.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Role of exercise and rapamycin on the expression of energy metabolism genes in liver tissues of rats fed a high?fat diet
    Authors: G Tu, C Dai, H Qu, Y Wang, B Liao
    Molecular Medicine Reports, 2020-07-28;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. C16 Peptide Promotes Vascular Growth and Reduces Inflammation in a Neuromyelitis Optica Model
    Authors: H Chen, X Fu, J Jiang, S Han
    Front Pharmacol, 2019-12-03;10(0):1373.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Bupivacaine at clinically relevant concentrations induces toxicity in human intervertebral disc cells via the induction of autophagy in vitro
    Authors: G Yang, Z Li, H Mei, W Ye, S Huang, K Liu, Q Tan
    Mol Med Rep, 2019-05-22;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Woodchuck VEGF (wVEGF) characteristics: Model for angiogenesis and human hepatocellular carcinoma directed therapies
    Authors: H Huang, O Salavaggio, L Rivera, S Mukherjee, R Brekken, B Tennant, R Iyer, A Adjei
    Arch. Biochem. Biophys., 2018-11-13;0(0):.
    Species: Primate
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Phosphoinositide 3-kinase-delta could be a biomarker for eosinophilic nasal polyps
    Authors: JS Kim, JS Jeong, KB Lee, SR Kim, YH Choe, SH Kwon, SH Cho, YC Lee
    Sci Rep, 2018-10-30;8(1):15990.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. Differential expression of ABCB5 in BRAF inhibitor-resistant melanoma cell lines
    Authors: J Xiao, ME Egger, KM McMasters, H Hao
    BMC Cancer, 2018-06-22;18(1):675.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Signaling Lymphocyte Activation Molecule Family 5 Enhances Autophagy and Fine-Tunes Cytokine Response in Monocyte-Derived Dendritic CellsStabilization of Interferon Regulatory Factor 8
    Authors: Z Agod, K Pazmandi, D Bencze, G Vereb, T Biro, A Szabo, E Rajnavolgy, A Bacsi, P Engel, A Lanyi
    Front Immunol, 2018-01-26;9(0):62.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  13. Modeling Multiple Myeloma-Bone Marrow interactions and response to drugs in a 3D surrogate microenvironment
    Authors: D Belloni, S Heltai, M Ponzoni, A Villa, B Vergani, L Pecciarini, M Marcatti, S Girlanda, G Tonon, F Ciceri, F Caligaris-, M Ferrarini, E Ferrero
    Haematologica, 2018-01-11;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Subthalamic Nucleus Deep Brain Stimulation Employs trkB Signaling for Neuroprotection and Functional Restoration
    Authors: DL Fischer, CJ Kemp, A Cole-Strau, NK Polinski, KL Paumier, JW Lipton, K Steece-Col, TJ Collier, DJ Buhlinger, CE Sortwell
    J. Neurosci., 2017-06-12;37(28):6786-6796.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Different dynamics of NLRP3 inflammasome-mediated IL?1? production in GM-CSF- and M-CSF-differentiated human macrophages
    Authors: MM Budai, J T?zsér, S Benk?
    J. Leukoc. Biol, 2017-02-23;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Ocular Delivery of PACAP1-27 Protects the Retina From Ischemic Damage in Rodents
    Invest. Ophthalmol. Vis. Sci, 2016-12-01;57(15):6683-6691.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  17. Dual inhibition of CDK4/Rb and PI3K/AKT/mTOR pathways by ON123300 induces synthetic lethality in mantle cell lymphomas.
    Authors: Divakar S, Ramana Reddy M, Cosenza S, Baker S, Perumal D, Antonelli A, Brody J, Akula B, Parekh S, Premkumar Reddy E
    Leukemia, 2015-07-15;30(1):86-93.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Interferon-gamma suppresses intestinal epithelial aquaporin-1 expression via Janus kinase and STAT3 activation.
    Authors: Dicay M, Hirota C, Ronaghan N, Peplowski M, Zaheer R, Carati C, MacNaughton W
    PLoS ONE, 2015-03-20;10(3):e0118713.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Water-filtered infrared a irradiation in combination with visible light inhibits acute chlamydial infection.
    Authors: Marti H, Koschwanez M, Pesch T, Blenn C, Borel N
    PLoS ONE, 2014-07-14;9(7):e102239.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Neuronal pentraxin 2 supports clear cell renal cell carcinoma by activating the AMPA-selective glutamate receptor-4.
    Authors: von Roemeling C, Radisky D, Marlow L, Cooper S, Grebe S, Anastasiadis P, Tun H, Copland J
    Cancer Res, 2014-06-24;74(17):4796-810.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. The role of nuclear beta-catenin accumulation in the Twist2-induced ovarian cancer EMT.
    Authors: Mao Y, Xu J, Li Z, Zhang N, Yin H, Liu Z
    PLoS ONE, 2013-11-11;8(11):e78200.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Single amino Acid substitutions in the chemotactic sequence of urokinase receptor modulate cell migration and invasion.
    Authors: Bifulco, Katia, Longanesi-Cattani, Immacola, Franco, Paola, Pavone, Vincenzo, Mugione, Pietro, Di Carluccio, Gioconda, Masucci, Maria Te, Arra, Claudio, Pirozzi, Giuseppe, Stoppelli, Maria Pa, Carriero, Maria Vi
    PLoS ONE, 2012-09-25;7(9):e44806.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. An elaborate regulation of Mammalian target of rapamycin activity is required for somatic cell reprogramming induced by defined transcription factors.
    Authors: He, Jing, Kang, Lan, Wu, Tong, Zhang, Jiqin, Wang, Haitao, Gao, Haibo, Zhang, Yu, Huang, Bo, Liu, Wenqiang, Kou, Zhaohui, Zhang, Hongbing, Gao, Shaorong
    Stem Cells Dev, 2012-05-17;21(14):2630-41.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. EphB receptors trigger Akt activation and suppress Fas receptor-induced apoptosis in malignant T lymphocytes.
    Authors: Maddigan A, Truitt L, Arsenault R
    J. Immunol., 2011-10-28;187(11):5983-94.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt.
    Authors: Deng J, Han Y, Yan C, Tian X, Tao J, Kang J, Li S
    Apoptosis, 2010-04-01;15(4):463-73.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation.
    Authors: Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, Kang J, Zhang X, Yan C
    J. Mol. Cell. Cardiol., 2010-01-06;48(6):1225-35.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. CREG inhibits migration of human vascular smooth muscle cells by mediating IGF-II endocytosis.
    Authors: Han Y, Cui J, Tao J, Guo L, Guo P, Sun M, Kang J, Zhang X, Yan C, Li S
    Exp. Cell Res., 2009-09-19;315(19):3301-11.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Osteoblast-derived factors induce an expression signature that identifies prostate cancer metastasis and hormonal progression.
    Authors: Wang G, Haile S, Comuzzi B, Tien AH, Wang J, Yong TM, Jelescu-Bodos AE, Blaszczyk N, Vessella RL, Masri BA, Sadar MD
    Cancer Res., 2009-04-07;69(8):3433-42.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. Enhancing mammalian target of rapamycin (mTOR)-targeted cancer therapy by preventing mTOR/raptor inhibition-initiated, mTOR/rictor-independent Akt activation.
    Authors: Wang X, Yue P, Kim YA, Fu H, Khuri FR, Sun SY
    Cancer Res., 2008-09-15;68(18):7409-18.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  30. The involvement of the fractalkine receptor in the transmigration of neuroblastoma cells through bone-marrow endothelial cells.
    Authors: Nevo I, Sagi-Assif O, Meshel T, Ben-Baruch A, Johrer K, Greil R, Trejo LE, Kharenko O, Feinmesser M, Yron I, Witz IP
    Cancer Lett., 2008-09-07;273(1):127-39.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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