Human/Mouse/Rat PI 3-Kinase p55 gamma Antibody

Name: Human/Mouse/Rat PI 3-Kinase p55 gamma Antibody
Brand: R&D Systems
SKU: AF5734
Price: 139 USD
Availability: InStock

R&D Systems | Catalog # AF5734

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all PI 3-Kinase p55 gamma Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PI 3-Kinase p55 gamma
Lys251-Gly378
Accession # Q92569

Specificity

Detects endogenous human, mouse and rat PI 3-Kinase p55 gamma in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat PI 3-Kinase p55 gamma Antibody

Detection of Human/Mouse/Rat PI 3-Kinase p55 gamma by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PI 3-Kinase p55 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5734) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PI 3-Kinase p55 gamma at approximately 55 to 57 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat PI 3-Kinase p55 gamma Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: SH-SY5Y human neuroblastoma cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PI 3-Kinase p55 gamma

p55 gamma (Protein of 55 kDa, gamma subunit; also PI 3-Kinase regulatory subunit gamma and p55^PIK) is a 55-57 kDa member of the PI 3K p85 family of regulatory subunits. It is widely expressed and serves as a recruitment subunit for the p110 catalytic subunit of PI 3-Kinase. p55 gamma /p55^PIK should not be confused with the 55 kDa alpha splice variant of the p85 alpha gene (75% amino acid [aa] identity). Human p55 gamma is 461 aa in length and contains a unique 34 aa N-terminus that binds Rb and prevents cycle progression, followed by a Pro-rich region (aa 35-44) and two SH2 domains that bind the p110 catalytic subunit (aa 65-160 and 358-452).