Detection of Human/Mouse/Rat PIM2 by Western Blot.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, Raji human Burkitt's lymphoma cell line, SW480 human colorectal adenocarcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and rat brain tissue. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat PIM2 Monoclonal Antibody (Catalog # MAB4355) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for PIM2 was detected at approximately 38 kDa and 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Detection of Human PIM2 by Western Blot.
Western blot shows Recombinant Human Active PIM1 (Catalog # 4588‑KS), recombinant human PIM2, and recombinant human PIM3 (5 ng/lane). PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/ Mouse/Rat PIM2 Monoclonal Antibody (Catalog # MAB4355) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for PIM2 was detected at approximately 38‑40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
The three members of the Pim family of proto-oncogenes encode the serine/threonine kinases PIM1, PIM2, and PIM3. Multiple isoforms of PIM2 are expressed in many tissues, with high levels in spleen, thymus, testis, small intestine, and colon. PIM2 is implicated as a mediator of IL-3 survival signaling in hematopoietic cells, and dysregulated PIM2 expression is observed in prostate cancer, lymphoma, leukemia, and multiple myeloma.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 -
Role of PIM2 in allergic asthma
Authors: W Du, T Chen, Y Ni, X Hou, Y Yu, Q Zhou, F Wu, W Tang, G Shi
Mol Med Rep, 2017;0(0):.
Sample Type: Whole Cells
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