PKM2 Antibody (945131)

R&D Systems | Catalog # MAB72442

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 945131
View all PKM2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PKM2
Ser2-Pro531
Accession # P14618-1

Specificity

Detects human PKM2 in direct ELISAs. Detects human, mouse and rat PKM1/2 in Western blot.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for PKM2 Antibody (945131)

Detection of Human, Mouse, and Rat PKM2 antibody by Western Blot.

Detection of Human, Mouse, and Rat PKM2 by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line, BaF3 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat PKM1/2 Monoclonal Antibody (Catalog # MAB72442) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human PKM2 by Western Blot.

Western blot shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line, A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, human skeletal muscle. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat PKM2 Monoclonal Antibody (Catalog # MAB72442) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for PKM2 Antibody (945131)

Application
Recommended Usage

Western Blot

0.25-0.5 µg/mL
Sample: A549 human lung carcinoma cell line, BaF3 mouse pro-B cell line, and Rat‑2 rat embryonic fibroblast cell line, U‑87 MG human glioblastoma/astrocytoma cell line, HeLa human cervical epithelial carcinoma cell line, human skeletal muscle

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PKM2

PKM2 (Pyruvate Kinase isoenzyme M2; also p58, OIP3, THBP1 and CTHBP) is a 58-60 kDa member of the PK family of enzymes. It is widely expressed, being found both intracellularly and in blood, and represents the more common splice variant of the PKM gene. PKM2 generates ATP and pyruvate by catalyzing the transfer of a phosphoryl group from PEP to ADP. Thus, when active, PKM2 promotes energy production and glycolysis. PKM2 exists as a marginally active monomer, with full activity achieved through homotetramerization. Notably, in tumor cells, select oncogenes appear to induce PKM2 homodimerization which limits PKM2 activity. PKM2 is known to be regulated by the binding of T3 and Fru-1,6-bisP. Human PKM2 is 531 amino acids (aa) in length. It contains a catalytic region (aa 43‑527) plus four utilized Ser/Thr and Tyr phosphorylation sites, respectively. PKM1 is another PKM gene splice variant that shows a 45 aa substitution for aa 389‑433 of PKM2. This variant shows limited expression (striated muscle) and hyperbolic Michaelis-Menten kinetics. There are additional isoform variants of PKM2 that show either a deletion of aa 59‑132, or a 67 aa substitution for aa 1‑82. Over aa 434‑531, human PKM2 shares 95% aa sequence identity with mouse PKM2.

Long Name

Pyruvate Kinase, Muscle

Alternate Names

CTHBP, OIP3, p58, PK2, PK3, TCB, THBP1, Tumor M2-PK

Entrez Gene IDs

5315 (Human); 18746 (Mouse); 25630 (Rat)

Gene Symbol

PKM

UniProt

P14618-1

Additional PKM2 Products

Product Documents for PKM2 Antibody (945131)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PKM2 Antibody (945131)

For research use only

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FAQs

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Associated Pathways

HIF Enhancer Pathways
HIF Enhancer Pathway Thumbnail