Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 455410
View all Rap2A/B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Rap2A/B
Met1-Gln183
Accession # P10114

Specificity

Detects human, mouse, and rat Rap2A/B in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat Rap2A/B Antibody

Detection of Human, Mouse, and Rat Rap2A/B antibody by Western Blot.

Detection of Human, Mouse, and Rat Rap2A/B by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat Rap2A/B Monoclonal Antibody (Catalog # MAB4698) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Rap2A and Rap2B (5 ng/lane) were included. A specific band for Rap2A/B was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human/Mouse/Rat Rap2A/B Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Rap2A/B

Rap2A and Rap2B are members of the small GTPase superfamily and ras family of proteins. Both proteins are 183 amino acids (aa) in length (SwissProt P10114 and P61225), and they are 90% identical at the amino acid level, with the most variability at the carboxyl terminus of the protein. Unlike normal Ras proteins that have a nontransforming glutamine at aa 61, these two oncogenic proteins have a threonine in that position. This change makes the intrinsic GTPase activity for these proteins lower, thereby allowing them to exist in the activated state for a longer period of time than normal Ras proteins.

Long Name

Ras-related Protein 2A/B

Alternate Names

KREV;K-REV;RAP2;Ras-related protein Rap-2a;RbBP-30

UniProt

P10114

Additional Rap2A/B Products

Product Documents for Human/Mouse/Rat Rap2A/B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Rap2A/B Antibody

For research use only

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