Detection of Human, Mouse, and Rat RSK by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat RSK Pan Specific Monoclonal Antibody (Catalog # MAB2056) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for RSK at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for RSK at approximately 86 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat RSK Pan Specific Monoclonal Antibody (Catalog # MAB2056). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
The 90 kDa ribosomal protein S6 kinases
(RSK1-4) are a family of broadly expressed Ser/Thr kinases activated in
response to mitogenic stimuli, including growth factors and tumor-promoting
phorbol esters. RSKs are directly phosphorylated by ERK1 and ERK2, leading to
RSK autophosphorylation and activation. Active RSKs appear to play a major role
in transcriptional regulation by translocating to the nucleus and
phosphorylating c-Fos and CREB. Within the range used as immunogen, human RSK2
shares 96%, 100%, and 91% amino acid sequence identity with RSK1, RSK3, RSK4,
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