SHP-2 Antibody

Detection of Human/Mouse/Rat SHP‑2 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HT-29 human colon adenocarcinoma cell line, Raji human Burkitt's lymphoma cell line, and NR-6 mouse fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL Goat Anti-Human/Mouse/Rat SHP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1894) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human Active SHP-1 aa 205-595 (Catalog # 1878-SH) and Recombinant Human SHP-2 (Catalog # 1894-SH) (2 ng/lane) were included. A specific band for SHP-2 was detected at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Shows Human SHP‑2 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and SHP-2 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat SHP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1894) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SHP-2 at approximately 80 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of SHP-2 by Western Blot

SFX-01 inhibits wild-type and mutant Shp2 phosphatase activity in vivo.Shp2 phosphatase activity in (A) cardiac tissue (n = 8 mice) and (B) spleen of WT (n = 4 mice) or Ptpn11D61G(−/+) mice (n = 5 mice) that received SFX-01 in the drinking water for 10 days. (A, B) Bars represent mean activity ( ± SEM) and P values calculated by two-way ANOVA with Sîdak post hoc test. (C) Representative immunoblots showing the input and immunoprecipitated Shp2 protein and SFN-labeled Shp2 from cardiac tissue isolated from mice with or without oral SFX-01 administration as in (A). (D) Representative immunoblot of Shp2 immunoprecipitated from cardiac tissue of WT mice following treatment with SFX-01 for 4 days after incubation with biotin-iodoacetamide. (E) Representative far-western immunoblot of Shp2 immunoprecipitated from cardiac tissue of WT mice following treatment with SFX-01 for 4 days. The immunoblot was then exposed to biotin-phenylarsenic acid (PAA). Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (https://link.springer.com/10.1038/s44321-025-00267-7), licensed under a CC-BY license. Not internally tested by R&D Systems.