Detection of STAT6 in HeLa Human Cell Line by Flow Cytometry.
HeLa human cervical epithelial carcinoma cell line was stained with Goat Anti-Human/Mouse/Rat STAT6 aa 627‑838 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2167, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
Detection of Mouse STAT6 aa 627-838 by Western Blot.
Western blot shows lysates of DA3 mouse myeloma cell line untreated (-) or treated (+) with 25 μg Recombinant Mouse IL-4 (Catalog # 404-ML) for 30 minutes. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat STAT6 Polyclonal Antibody (Catalog # AF2167, followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for STAT6 aa 627-838 at approximately 100 - 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Signal Transducer and Activator of Transcription 6 (STAT6) is a member of the Jak/STAT signal transduction pathway and mediates cytokine signaling by IL-4. IL-4 activation leads to tyrosine phosphorylation and accumulation in the nucleus of STAT6 homo and heterodimers. STAT6 acts as a signal transducer in the cytoplasm and, upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN4GAA. Knockout studies in mice suggest that STAT6 functions in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. STAT6 transcriptional activation occurs by binding p100 and p160 class (NcoA1, Src-1) co-activators. Gene expression studies indicate that STAT6 mediates cell proliferation and anti-apoptotic effects of IL-4.
Signal Transducer and Activator of Transcription 6
Entrez Gene IDs:
6778 (Human); 20852 (Mouse); 362896 (Rat)
D12S1644; EC 220.127.116.11; EC 18.104.22.168; IL-4 Stat; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin4-induced; STAT6; STAT6B; STAT6C; transcription factor IL-4 STAT
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