Syntaxin-BP1 in Mouse Brain.
Syntaxin-BP1 was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Human/Mouse/Rat Syntaxin-BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5675) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Human/Mouse/Rat Syntaxin-BP1 by Western Blot.
Western blot shows lysates of rat brain tissue and spinal cord tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Syntaxin-BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5675) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Syntaxin-BP1 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
STXBP1 (Syntaxin-binding protein 1; also p67, Munc18-1, N-Sec1 and Unc18A) is a 67 kDa member of the SNARE-interacting Sec1/Unc18/STXBP family of proteins. It is expressed in a variety of cells, presumably in those that exhibit granule exocytosis, and likely participates in (synaptic) granule release. Ligands for STXBP1 include STX1A, 2 and 3, and it is possible that STXBP1 both suppresses STX activity, and serves as an anchor for STX-mediated vesicle fusion. Human STXBP1 is 594 amino acids (aa) in length. It contains one SEC1 domain (aa 41‑575). Phosphorylation at Ser313 may stimulate granule release. There is one splice variant that shows a 28 aa substitution for aa 576‑594. This is restricted to neural tissue. Over aa 80‑226, human and mouse STXBP1 are absolutely identical in amino acid sequence.
Syntaxin-binding Protein 1
Entrez Gene IDs:
6812 (Human); 20910 (Mouse); 25558 (Rat)
EIEE4; FLJ37475; hUNC18; MUNC18-1; NSEC1; N-Sec1; p67; Protein unc-18 homolog 1; Protein unc-18 homolog A; rbSec1; STXBP1; syntaxin binding protein 1; syntaxin-binding protein 1; SyntaxinBP1; Syntaxin-BP1; UNC18; unc18-1; UNC18A; unc-18A; UNC18neuronal SEC1
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