Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Syntaxin-BP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Syntaxin-BP1
Ser80-Ala226
Accession # P61764

Specificity

Detects human, mouse, and rat Syntaxin-BP1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Syntaxin-BP2 and rhSyntaxin-BP3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Syntaxin‑BP1 Antibody

Syntaxin-BP1 antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

Syntaxin-BP1 in Mouse Brain.

Syntaxin-BP1 was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Human/Mouse/Rat Syntaxin-BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5675) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Human/Mouse/Rat Syntaxin-BP1 antibody by Western Blot.

Detection of Human/Mouse/Rat Syntaxin-BP1 by Western Blot.

Western blot shows lysates of rat brain tissue and spinal cord tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Syntaxin-BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5675) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Syntaxin-BP1 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Western Blot Shows Syntaxin-BP1 Specificity Using Knockout Cell Line.

Western Blot Shows Syntaxin‑BP1 Specificity Using Knockout Cell Line.

Western blot shows lysates of U‑87 MG human glioblastoma/astrocytoma parental cell line and Syntaxin‑BP1 knockout U-87 MG cell line (KO). Nitrocellulose membrane was probed with 1 ug/mL of Goat Anti-Human/Mouse/Rat Syntaxin‑BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5675) followed by HRP-conjugated secondary antibody. A specific band was detected for Syntaxin‑BP1 at approximately 67 kDa (as indicated) in the parental U-87 MG cell line, but is not detectable in knockout U-87 MG cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.
Detection of Syntaxin-BP1 by Immunoprecipitation.

Detection of Syntaxin‑BP1 by Immunoprecipitation.

U‑87 MG human glioblastoma/astrocytoma cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of Goat Anti-Human/Mouse/Rat Syntaxin‑BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5675) pre-coupled to Dynabeads Protein G. Immunoprecipitated Syntaxin‑BP1 was detected in Western Blot with a Rabbit Syntaxin-BP1 antibody. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Syntaxin-BP1 Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

Syntaxin‑BP1 Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

U‑87 MG human glioblastoma/astrocytoma parental cell line WT and Syntaxin‑BP1 U-87 MG KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Goat Anti-Human/Mouse/Rat Syntaxin‑BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5675) followed by incubation with an Alexa-fluor 555 conjugated secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Primary antibody concentration used: 1 ug/mL. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Applications for Human/Mouse/Rat Syntaxin‑BP1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (hippocampus)

Immunoprecipitation

2 µg/mL
Sample: Cell lysate of U-87 MG human glioblastoma/astrocytoma cell line

Knockout Validated

A specific band was detected for Syntaxin‑BP1 at approximately 67 kDa (as indicated) in the parental U-87 MG cell line, but is not detectable in knockout U-87 MG cell line.

Western Blot

1 µg/mL
Sample: Rat brain tissue and spinal cord tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Syntaxin-BP1

STXBP1 (Syntaxin-binding protein 1; also p67, Munc18-1, N-Sec1 and Unc18A) is a 67 kDa member of the SNARE-interacting Sec1/Unc18/STXBP family of proteins. It is expressed in a variety of cells, presumably in those that exhibit granule exocytosis, and likely participates in (synaptic) granule release. Ligands for STXBP1 include STX1A, 2 and 3, and it is possible that STXBP1 both suppresses STX activity, and serves as an anchor for STX-mediated vesicle fusion. Human STXBP1 is 594 amino acids (aa) in length. It contains one SEC1 domain (aa 41‑575). Phosphorylation at Ser313 may stimulate granule release. There is one splice variant that shows a 28 aa substitution for aa 576‑594. This is restricted to neural tissue. Over aa 80‑226, human and mouse STXBP1 are absolutely identical in amino acid sequence.

Long Name

Syntaxin-binding Protein 1

Alternate Names

EIEE4, p67, rbSec1, STXBP1, SyntaxinBP1, UNC18

Entrez Gene IDs

6812 (Human); 20910 (Mouse); 25558 (Rat)

Gene Symbol

STXBP1

UniProt

P61764

Additional Syntaxin-BP1 Products

Product Documents for Human/Mouse/Rat Syntaxin‑BP1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Syntaxin‑BP1 Antibody

For research use only

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Protocols

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