Detection of Human and Mouse ROR gamma /NR1F3 by Western Blot. |
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, EL‑4 mouse lymphoblast cell line, and mouse thymus tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse ROR gamma /NR1F3 Monoclonal Antibody (Catalog # MAB6109) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ROR gamma /NR1F3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ROR gamma (Nuclear receptor ROR-gamma), also called RORC and NR1F3, is a member of the NR1 nuclear hormone receptor family. ROR gamma is a DNA binding transcription factor. ROR gamma is 518 amino acids (aa) in length. Deletion in mice implicates ROR gamma as being essential for lymphoid organogenesis and controlling apoptosis during thymopoiesis. Two splice forms differing in the first 24 aa have been found for this gene; isoform 2 deletes aa 1‑21 resulting in an alteration of aa 22‑24. Over aa 1‑100 human RORC shares 96% identity with mouse ROR gamma.
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