Human/Mouse ROR gamma /RORC/NR1F3 Antibody

Catalog # Availability Size / Price Qty
MAB6109
MAB6109-SP
Detection of Human and Mouse ROR gamma /NR1F3 by Western Blot.
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (2)

Human/Mouse ROR gamma /RORC/NR1F3 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse ROR gamma /RORC/NR1F3 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 600214
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human ROR gamma /RORC/NR1F3
Met1-Gln100
Accession # P51449-2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse ROR?/NR1F3 antibody by Western Blot. View Larger

Detection of Human and Mouse ROR gamma /NR1F3 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, EL-4 mouse lymphoblast cell line, and mouse thymus tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse ROR gamma/NR1F3 Monoclonal Antibody (Catalog # MAB6109) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ROR gamma/NR1F3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROR gamma/RORC/NR1F3

ROR gamma (Nuclear receptor ROR-gamma), also called RORC and NR1F3, is a member of the NR1 nuclear hormone receptor family. ROR gamma is a DNA binding transcription factor. ROR gamma is 518 amino acids (aa) in length. Deletion in mice implicates ROR gamma as being essential for lymphoid organogenesis and controlling apoptosis during thymopoiesis. Two splice forms differing in the first 24 aa have been found for this gene; isoform 2 deletes aa 1‑21 resulting in an alteration of aa 22‑24. Over aa 1‑100 human RORC shares 96% identity with mouse ROR gamma.

Long Name
Retinoic Acid-Related Orphan Receptor gamma
Entrez Gene IDs
6097 (Human)
Alternate Names
MGC129539; NR1F3; Nuclear receptor ROR gamma; Nuclear receptor ROR-gamma; Nuclear receptor RZR gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; RAR-Related Orphan Nuclear Receptor Variant 2; RAR-related orphan receptor C; RAR-related orphan receptor gamma; retinoic acid-binding receptor gamma; retinoid-related orphan receptor gamma; Retinoid-related orphan receptor-gamma; ROR gamma; RORC; RORG; RORGMGC129539; RZRG; RZRGRZR-GAMMA; TOR

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Citations for Human/Mouse ROR gamma /RORC/NR1F3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The YAP/HIF-1&alpha/miR-182/EGR2 axis is implicated in asthma severity through the control of Th17 cell differentiation
    Authors: J Zhou, N Zhang, W Zhang, C Lu, F Xu
    Cell & bioscience, 2021;11(1):84.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Soluble PD-L1 improved direct ARDS by reducing monocyte-derived macrophages
    Authors: J Xu, J Wang, X Wang, R Tan, X Qi, Z Liu, H Qu, T Pan, Q Zhan, Y Zuo, W Yang, J Liu
    Cell Death Dis, 2020;11(10):934.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: CyTof
  3. TLR2 Stimulation Drives Human Naive and Effector Regulatory T Cells into a Th17-Like Phenotype with Reduced Suppressive Function.
    Authors: Nyirenda MH, Sanvito L, Darlington PJ
    J. Immunol., 2011;187(5):2278-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Isotype Controls

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Secondary Antibodies

Reviews for Human/Mouse ROR gamma /RORC/NR1F3 Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human/Mouse ROR gamma /RORC/NR1F3 Antibody
By Anonymous on 12/20/2021
Application: WB Sample Tested: Thymus tissue Species: Mouse

Human/Mouse ROR gamma /RORC/NR1F3 Antibody
By Anonymous on 12/05/2020
Application: Flow Sample Tested: immune cells from lung Species: Mouse