Signal transduction and activator of transcription 4 (STAT4) is a 86 kDa member of the Jak/STAT signal transduction pathway. Human STAT4 is 748 amino acids (aa) in length. STAT4 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, homo- or heterodimerizes with other STAT proteins, which then translocate to the nucleus and activate transcription of specific genes. STAT4 contains a SH2 domain (aa 569‑664) and a Jak1 phophorylation site at Y693. STAT4 is involved in mediating IL‑12 response in lymphocytes and regulating T helper cell differentiation, and STAT4 variants are found in patients with systemic lupus erythematosus.
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 513710
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Product Specifications
Immunogen
E. coli-derived recombinant human STAT4
Leu83-Lys162
Accession # Q14765
Leu83-Lys162
Accession # Q14765
Specificity
Detects endogenous human STAT4 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse STAT4 Antibody
Detection of Human STAT4 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and NC-37 human Burkitt's lymphoma B lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse STAT4 Monoclonal Antibody (Catalog # MAB5287) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STAT4 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Applications for Human/Mouse STAT4 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and NC-37 human Burkitt's lymphoma B lymphoblast cell line
Sample: Jurkat human acute T cell leukemia cell line and NC-37 human Burkitt's lymphoma B lymphoblast cell line
Reviewed Applications
Read 3 reviews rated 4 using MAB5287 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: STAT4
Long Name
Signal Transducer and Activator of Transcription 4
Alternate Names
signal transducer and activator of transcription 4, SLEB11
Gene Symbol
STAT4
UniProt
Additional STAT4 Products
Product Documents for Human/Mouse STAT4 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse STAT4 Antibody
For research use only
Related Research Areas
Citations for Human/Mouse STAT4 Antibody
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Customer Reviews for Human/Mouse STAT4 Antibody (3)
4 out of 5
3 Customer Ratings
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 03/11/2019
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Application: ELISASample Tested: PlasmaSpecies: HumanVerified Customer | Posted 11/10/2018
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Application: MicroarraySample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 11/09/2018
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
