Detection of Human STAT4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and NC‑37 human Burkitt's lymphoma B lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse STAT4 Monoclonal Antibody (Catalog # MAB5287) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STAT4 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Signal transduction and activator of transcription 4 (STAT4) is a 86 kDa member of the Jak/STAT signal transduction pathway. Human STAT4 is 748 amino acids (aa) in length. STAT4 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, homo- or heterodimerizes with other STAT proteins, which then translocate to the nucleus and activate transcription of specific genes. STAT4 contains a SH2 domain (aa 569‑664) and a Jak1 phophorylation site at Y693. STAT4 is involved in mediating IL‑12 response in lymphocytes and regulating T helper cell differentiation, and STAT4 variants are found in patients with systemic lupus erythematosus.
Signal Transducer and Activator of Transcription 4
Entrez Gene IDs:
6775 (Human); 20849 (Mouse)
signal transducer and activator of transcription 4; SLEB11; STAT4
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