Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Frozen, Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 259820
View all TSG-6 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TSG-6
Trp18-Leu277
Accession # P98066

Specificity

Detects human and mouse TSG-6 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse TSG‑6 Antibody

Detection of TSG-6 by Western Blot

Detection of TSG-6 by Western Blot

Phosphorylated beta -catenin at S311 by PLK1 induced metastasis in an in vivo model. A549 cells expressing RFP-tagged WT of beta -catenin and WT at S311, S311D, or S311A of beta -cateninmtGSK3 beta were injected intravenously into the tail veins of four-week-old BALB/c nude mice, and the tumorigenic and metastatic properties were evaluated after 15 weeks. A, Representative lung tumors from the mouse model. B, The number of metastatic lung tumors was counted and plotted (n = 5). Data are presented as mean ± SD. C-F, Representative H&E staining (C-D) and Ki-67 staining (E-F) were performed using lung tissue from the mice. The relative density of H&E staining (D) and Ki-67 staining (F) was analyzed and plotted. *p <0.05; **p <0.01; ***p <0.001. Data are presented as mean ± SD. G, Immunoblotting was performed using lung tissue lysates from each mouse model. beta -catenin, RFP, N-cadherin, vimentin, CD44, c-Jun, TSG6, laminin gamma 2, and beta -actin were detected using specific antibodies. The band intensity values were quantified using densitometry of Photoshop software, normalized, and plotted (left panel). *p <0.05; **p <0.01; ***p <0.001. H, qRT-PCR was performed for RFP, CDH1, CDH2, CD44, JUN, LAMC2, and TNFAIP6 using lung tissue lysates from each mouse model. The relative mRNA expression was plotted. *p <0.05; **p <0.01; ***p <0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36793862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of TSG-6 by Western Blot

Detection of TSG-6 by Western Blot

Clinical relevance of PLK1 and beta -catenin in metastatic NSCLC. A-C, The overall survival (OS) times in NSCLC whole patients (n=1292) (A), NSCLC patients with stage 1 (n=522) (B), and stages 3-4 (n=48) (C) were analyzed according to their PLK1 and CTNNB1 expression levels using KM PLOTTER. High (Hi) and low (Lo) were generated by dividing patients according to their expression at the median cut-off. D, A heatmap analysis was performed from the TCGA dataset of lung adenocarcinoma patients for CTNNB1, PLK1, TNFAIP6, LAMC2, CD44, epithelial marker (CDH1), and mesenchymal markers (CDH2, SNAI1, SNAI2, TWIST1, TWIST2, ZEB1, and ZEB2) in paired normal (left) and tumor tissues (right) depending on stages. E-F, A549 and NCI-H460 (H460) non-small cell lung cancer cells were treated with 2.5 ng/ml of TGF-beta for 48 hours. E, Immunoblotting was performed to measure the protein levels of beta -catenin, laminin gamma 2, TSG6, CD44, c-Jun, c-Fos, PLK1, p-PLK1T210, p-Smad2S465/S467, Smad2/3, E-cadherin, N-cadherin, vimentin, SNAI1, SNAI2, and GAPDH in A549 (left panel) and NCI-H460 (H460) cells (right panel). F, The relative band intensities for beta -catenin, laminin gamma 2, CD44, TSG6, c-Jun, c-Fos, PLK1, p-PLK1T210, E-cadherin, N-cadherin, vimentin, SNAI1, and SNAI2 were quantified using densitometry of Photoshop software. G, The relative band intensities for beta -catenin/GAPDH, p-PLK1T210/PLK1, and p-Smad2S465/S467/Smad2 were quantified using densitometry of Photoshop software. H-I, qRT-PCR was performed for CTNNB1, CDH1, CDH2, PLK1, TNFAIP6, LAMC2, and CD44 expression in A549 (H) and NCI-H460 (I) cells. Data are presented as mean ± SD of at least three independent experiments (significantly different as compared with experimental control). *p <0.05; **p <0.01; ***p <0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36793862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of TSG-6 by Western Blot

Detection of TSG-6 by Western Blot

Phosphorylated beta -catenin at S311 by PLK1 induced metastasis in an in vivo model. A549 cells expressing RFP-tagged WT of beta -catenin and WT at S311, S311D, or S311A of beta -cateninmtGSK3 beta were injected intravenously into the tail veins of four-week-old BALB/c nude mice, and the tumorigenic and metastatic properties were evaluated after 15 weeks. A, Representative lung tumors from the mouse model. B, The number of metastatic lung tumors was counted and plotted (n = 5). Data are presented as mean ± SD. C-F, Representative H&E staining (C-D) and Ki-67 staining (E-F) were performed using lung tissue from the mice. The relative density of H&E staining (D) and Ki-67 staining (F) was analyzed and plotted. *p <0.05; **p <0.01; ***p <0.001. Data are presented as mean ± SD. G, Immunoblotting was performed using lung tissue lysates from each mouse model. beta -catenin, RFP, N-cadherin, vimentin, CD44, c-Jun, TSG6, laminin gamma 2, and beta -actin were detected using specific antibodies. The band intensity values were quantified using densitometry of Photoshop software, normalized, and plotted (left panel). *p <0.05; **p <0.01; ***p <0.001. H, qRT-PCR was performed for RFP, CDH1, CDH2, CD44, JUN, LAMC2, and TNFAIP6 using lung tissue lysates from each mouse model. The relative mRNA expression was plotted. *p <0.05; **p <0.01; ***p <0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36793862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of TSG-6 by Western Blot

Detection of TSG-6 by Western Blot

Clinical relevance of PLK1 and beta -catenin in metastatic NSCLC. A-C, The overall survival (OS) times in NSCLC whole patients (n=1292) (A), NSCLC patients with stage 1 (n=522) (B), and stages 3-4 (n=48) (C) were analyzed according to their PLK1 and CTNNB1 expression levels using KM PLOTTER. High (Hi) and low (Lo) were generated by dividing patients according to their expression at the median cut-off. D, A heatmap analysis was performed from the TCGA dataset of lung adenocarcinoma patients for CTNNB1, PLK1, TNFAIP6, LAMC2, CD44, epithelial marker (CDH1), and mesenchymal markers (CDH2, SNAI1, SNAI2, TWIST1, TWIST2, ZEB1, and ZEB2) in paired normal (left) and tumor tissues (right) depending on stages. E-F, A549 and NCI-H460 (H460) non-small cell lung cancer cells were treated with 2.5 ng/ml of TGF-beta for 48 hours. E, Immunoblotting was performed to measure the protein levels of beta -catenin, laminin gamma 2, TSG6, CD44, c-Jun, c-Fos, PLK1, p-PLK1T210, p-Smad2S465/S467, Smad2/3, E-cadherin, N-cadherin, vimentin, SNAI1, SNAI2, and GAPDH in A549 (left panel) and NCI-H460 (H460) cells (right panel). F, The relative band intensities for beta -catenin, laminin gamma 2, CD44, TSG6, c-Jun, c-Fos, PLK1, p-PLK1T210, E-cadherin, N-cadherin, vimentin, SNAI1, and SNAI2 were quantified using densitometry of Photoshop software. G, The relative band intensities for beta -catenin/GAPDH, p-PLK1T210/PLK1, and p-Smad2S465/S467/Smad2 were quantified using densitometry of Photoshop software. H-I, qRT-PCR was performed for CTNNB1, CDH1, CDH2, PLK1, TNFAIP6, LAMC2, and CD44 expression in A549 (H) and NCI-H460 (I) cells. Data are presented as mean ± SD of at least three independent experiments (significantly different as compared with experimental control). *p <0.05; **p <0.01; ***p <0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36793862), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of TSG-6 by Western Blot

Detection of TSG-6 by Western Blot

Reduced reproductive performance in old mice. A) Comparison of litter size in young and old mice. B) Decreased proliferation of granulosa cells in older mice. C–E) Cell cycle analysis in young and old mice. F–H) The mRNA (F) and protein (G-H) expression levels of CDK1, HAS2, TNFAIP6 and PTX3 in GCs. (n = 3). *P < 0.05, **P < 0.01 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39497025), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse TSG‑6 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human TSG‑6 (Catalog # 2104-TS)
Recombinant Mouse TSG‑6 (Catalog # 2326-TS)

Reviewed Applications

Read 10 reviews rated 4.1 using MAB2104 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TSG-6

TSG-6 is a secreted hyaluronan-binding protein composed of a link domain and Cub domain. It is induced in many cell types by inflammatory mediators. Via its link domain, TSG-6 is a potent inhibitor of polymorphonuclear leukocyte migration. TSG-6 forms a stable complex with the serine protease inhibitor Inter-alpha -Inhibitor (I alpha I) and potentiates the anti-plasmin activity of I alpha I.

Long Name

Tumor Necrosis Factor-stimulated Gene Sequence 6

Alternate Names

TNFAIP6, TSG6

Entrez Gene IDs

7130 (Human); 21930 (Mouse)

Gene Symbol

TNFAIP6

UniProt

P98066

Additional TSG-6 Products

Product Documents for Human/Mouse TSG‑6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse TSG‑6 Antibody

For research use only

Citations for Human/Mouse TSG‑6 Antibody

Customer Reviews for Human/Mouse TSG‑6 Antibody (10)

4.1 out of 5
10 Customer Ratings
5 Stars
20%
4 Stars
70%
3 Stars
10%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 10 reviews Showing All
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  • Human/Mouse TSG-6 Antibody
    Name: Ricardo Malvicini
    Application: Western Blot
    Sample Tested: extracellular vesicles
    Species: Human
    Verified Customer | Posted 09/10/2021
    Human/Mouse TSG‑6 Antibody MAB2104
  • Human/Mouse TSG-6 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Plasma
    Species: Mouse
    Verified Customer | Posted 09/01/2021
    Human/Mouse TSG‑6 Antibody MAB2104
  • Human/Mouse TSG-6 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Brain (cerebral cortex)
    Species: Mouse
    Verified Customer | Posted 08/06/2021
    this antibody was detected in the mouse cortex at different timepoint. It works very good with very clear band.
    Human/Mouse TSG‑6 Antibody MAB2104
  • Human/Mouse TSG-6 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human/Mouse TSG‑6 Antibody MAB2104
  • Human/Mouse TSG-6 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/21/2019
  • Human/Mouse TSG-6 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 01/19/2019
    Western Blot with diff concentration
    Human/Mouse TSG‑6 Antibody MAB2104
  • Human/Mouse TSG-6 Antibody
    Name: Simon Liu
    Application: Immunohistochemistry
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 12/08/2018
    Human/Mouse TSG‑6 Antibody MAB2104
  • Human/Mouse TSG-6 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/11/2018
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 22351758
    Species: Human
    Verified Customer | Posted 02/19/2015
  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: See PMID 22351758
    Species: Human
    Verified Customer | Posted 02/19/2015

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