ZAG Antibody (842014)

R&D Systems | Catalog # MAB47641

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 842014
View all ZAG Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ZAG
Met1-Ser298
Accession # P25311

Specificity

Detects human ZAG in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for ZAG Antibody (842014)

ZAG antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

ZAG in MCF‑7 Human Cell Line.

ZAG was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human ZAG Monoclonal Antibody (Catalog # MAB47641) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
ZAG antibody in 3T3-L1 Mouse Cell Line by Immunocytochemistry (ICC).

ZAG in 3T3‑L1 Mouse Cell Line.

ZAG was detected in immersion fixed 3T3-L1 mouse embryonic fibroblast adipose-like cell line using Mouse Anti-Human ZAG Monoclonal Antibody (Catalog # MAB47641) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for ZAG Antibody (842014)

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line and 3T3-L1 mouse embryonic fibroblast adipose-like cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ZAG

ZAG (zinc-alpha 2-glycoprotein; also ZA2G) is a 40 kDa, secreted member of the MHC class I family of proteins. It is produced by adipocytes and various epithelial cells that generate exocrine-type secretions. ZAG is reported to stimulate lipid breakdown and thus may play a role in lipid homeostasis. Mature human ZAG is 278 amino acids (aa) in length. It contains one MHC class I antigen region (aa 26‑201) and a C2‑type Ig‑like domain (aa 207‑292). Two alternate splice forms exist; one shows a 66 aa substitution for the C‑terminal 30 aa, and a second shows a nine Lys substitution for aa 151‑298. Mature human ZAG is 60% aa identical to mouse ZAG.

Long Name

Zinc alpha-2-Glycoprotein

Alternate Names

AZGP1, ZA2G, ZNGP1

Entrez Gene IDs

563 (Human); 12007 (Mouse); 25294 (Rat)

Gene Symbol

AZGP1

UniProt

P25311

Additional ZAG Products

Product Documents for ZAG Antibody (842014)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for ZAG Antibody (842014)

For research use only

Related Research Areas

Cancer Biomarkers

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