Detection of Human NAIP by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human NAIP and human brain (cerebellum) tissue. PVDF Membrane was probed with 1 µg/mL of Human NAIP Monoclonal Antibody (Catalog # MAB829) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NAIP at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
NAIP in Human Brain.
NAIP was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Human NAIP Monoclonal Antibody (Catalog # MAB829) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Neuronal apoptosis inhibitory protein (NAIP; also
Baculoviral IAP repeat-containing protein 1) is a 160 kDa member of the
inhibitor of apoptosis family of proteins (also known as BIRC proteins). Human
NAIP is 1403 amino acids (aa) in length. It contains three distinct regions: an
N-terminal cluster of three baculoviral inhibitory repeat (BIR) domains, a
central nucleotide binding oligomerization domain (NOD), and a C-terminal
leucine-rich repeat (LRR) domain. Human
NAIP shares 68% aa identity with mouse NAIP.
NAIP is expressed in motor neurons, but not in sensory neurons. It is also expressed in the liver, placenta
and to a lesser extent in the spinal cord.
NAIP prevents motor neuron apoptosis, and defects in NAIP have been found
in individuals with spinal muscular atrophy.
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