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Human Neuroligin 3/NLGN3 Antibody

Catalog #: MAB60881 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB60881
MAB60881-SP

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Detection of Human Neuroligin 3/NLGN3 by Western Blot.
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Human Neuroligin 3/NLGN3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Neuroligin 3/NLGN3 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) Neuroligin 3v2 and recombinant rat (rr) Neuroligin 3v4 is observed. No cross-reactivity with rhNeuroligin 2, rhNeuroligin 4, rrNeuroligin 1, 1v2, or 2v2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 566205
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Neuroligin 3/NLGN3
Gln38-Ser709
Accession # NP_061850
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Neuroligin 3/NLGN3 antibody by Western Blot. View Larger

Detection of Human Neuroligin 3/NLGN3 by Western Blot. Western blot shows lysates of human brain (hippocampus) tissue. PVDF Membrane was probed with 2 µg/mL of Human Neuroligin 3/NLGN3 Monoclonal Antibody (Catalog # MAB60881) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Neuroligin 3/NLGN3 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuroligin 3/NLGN3

NLGN3 (Neuroligin 3; also gliotactin homolog) is a 110-114 kDa member of the type-B carboxyesterase/lipase family of proteins.  It is a neuronal transmembrane protein that forms Ca++-dependent intercellular junctions with short beta -neurexin isoforms.  This seems to contribute to both glutamatergic and GABAergic synapse formation.  Mutations in NLGN3 are associated with a reduction in protein expression and the occurrence of autism.  Mature human NLGN3 is an 811 amino acid (aa) type I transmembrane protein.  It contains a 672 aa extracellular domain (ECD) (aa 38-709), plus a 118 aa cytoplasmic region.  The ECD possesses a nonfunctional carboxyesterase domain (aa 41-625).  Multiple splice variants exist.  There is a deletion of aa 153-172 that may also be accompanied by an alternative start site at Met118, and a deletion of aa 153-192 that may also be accompanied by a five Lys substitution for aa 554-848.  Over aa 39-709, human NLGN3 shares 99% aa identity with mouse NLGN3.        

Entrez Gene IDs
54413 (Human)
Alternate Names
ASPGX1; AUTSX1; Gliotactin homolog; HNL3; KIAA1480HNL3; Neuroligin 3; neuroligin-3; NL3; NLGN3

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