Human Neuroligin 3/NLGN3 Antibody (566205)

R&D Systems | Catalog # MAB60881

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 566205
View all Neuroligin 3/NLGN3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Neuroligin 3/NLGN3
Gln38-Ser709
Accession # NP_061850

Specificity

Detects human Neuroligin 3/NLGN3 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) Neuroligin 3v2 and recombinant rat (rr) Neuroligin 3v4 is observed. No cross-reactivity with rhNeuroligin 2, rhNeuroligin 4, rrNeuroligin 1, 1v2, or 2v2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Neuroligin 3/NLGN3 Antibody (566205)

Detection of Human Neuroligin 3/NLGN3 antibody by Western Blot.

Detection of Human Neuroligin 3/NLGN3 by Western Blot.

Western blot shows lysates of human brain (hippocampus) tissue. PVDF Membrane was probed with 2 µg/mL of Human Neuroligin 3/NLGN3 Monoclonal Antibody (Catalog # MAB60881) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Neuroligin 3/NLGN3 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Neuroligin 3/NLGN3 Antibody (566205)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human brain (hippocampus) tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neuroligin 3/NLGN3

NLGN3 (Neuroligin 3; also gliotactin homolog) is a 110-114 kDa member of the type-B carboxyesterase/lipase family of proteins.  It is a neuronal transmembrane protein that forms Ca++-dependent intercellular junctions with short beta -neurexin isoforms.  This seems to contribute to both glutamatergic and GABAergic synapse formation.  Mutations in NLGN3 are associated with a reduction in protein expression and the occurrence of autism.  Mature human NLGN3 is an 811 amino acid (aa) type I transmembrane protein.  It contains a 672 aa extracellular domain (ECD) (aa 38-709), plus a 118 aa cytoplasmic region.  The ECD possesses a nonfunctional carboxyesterase domain (aa 41-625).  Multiple splice variants exist.  There is a deletion of aa 153-172 that may also be accompanied by an alternative start site at Met118, and a deletion of aa 153-192 that may also be accompanied by a five Lys substitution for aa 554-848.  Over aa 39-709, human NLGN3 shares 99% aa identity with mouse NLGN3.        

Alternate Names

ASPGX1, AUTSX1, HNL3, NL3, NLGN3

Entrez Gene IDs

54413 (Human)

Gene Symbol

NLGN3

UniProt

NP_061850

Additional Neuroligin 3/NLGN3 Products

Product Documents for Human Neuroligin 3/NLGN3 Antibody (566205)

Certificate of Analysis

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Product Specific Notices for Human Neuroligin 3/NLGN3 Antibody (566205)

For research use only

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