Human NFATC2 Antibody

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Detection of Human NFATC2 by Western Blot.
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Product Details
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Human NFATC2 Antibody Summary

Species Reactivity
Detects human NFATC2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human NFATC1 or C3 is observed.
Monoclonal Mouse IgG2B Clone # 639402
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human NFATC-2
Accession # Q13469
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human NFATC2 antibody by Western Blot. View Larger

Detection of Human NFATC2 by Western Blot. Western blot shows lysates of BJAB human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Human NFATC2 Monoclonal Antibody (Catalog # MAB6499) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NFATC2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFATC2

NFATC2 (Nuclear factor of activated T cells C2; also NFAT1 or NFAT2P) is a 135 kDa member of the NFAT family of transcription factors. NFATC2 is found in T cells and mast cells where it regulates cytokine transcription, Th2 cell differentiation, and cell cycle entry. The transactivation function of NFATC2 is regulated by phosphorylation at Ser53, Ser56, Thr116, and Ser170. Human NFATC2 is 925 amino acids (aa) in length. It contains a calcineurin-binding site (aa 111-116), a transactivation domain (aa 119-199), two NLS (aa 251-253 and 664-666), an RHD that binds DNA (aa 392-574), and one NES (aa 904-913). An alternate splice form has a substitution of the C-terminal 18 amino acids. Human NFATC2 shares 96% aa identity with mouse and rat NFATC2.

Long Name
Nuclear factor of activated T-cells, cytoplasmic 2
Entrez Gene IDs
4773 (Human)
Alternate Names
NFAT pre-existing subunit; NFAT transcription complex, preexisting component; NFAT1; NFAT1nuclear factor of activated T-cells, cytoplasmic 2; NFATC2; NF-ATc2; NFATP; NF-ATp; NFATPnuclear factor of activated T-cells, preexisting component; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2; preexisting nuclear factor of activated T-cells 2; T cell transcription factor NFAT1; T-cell transcription factor NFAT1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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