Human NFATC3 Antibody (646115)

R&D Systems | Catalog # MAB5834

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 646115
View all NFATC3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NFATC3
Asp900-Asp1035
Accession # Q12968

Specificity

Detects human NFATC3 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human NFATC3 Antibody (646115)

Detection of Human NFATC3 antibody by Western Blot.

Detection of Human NFATC3 by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Human NFATC3 Monoclonal Antibody (Catalog # MAB5834) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NFATC3 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human NFATC3 Antibody (646115)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NFATC3

NFATC3 (Nuclear factor of activated T cells C3; also NFAT4 and NFATx) is a 145-150 kDa member of the NFAT family of transcription factors. NFATC3 is widely expressed being found in DP thymocytes, CD4+ T cells, smooth muscle cells, Schwann cells and mast cells. NFATC3 regulates gene transcription as part of a NFATC transcription complex. It is normally cytoplasmic and phosphorylated. Upon a rise in intracellular Ca+2, dephosphorylation occurs via calcineurin, and NFATC3 enters the nucleus. Human NFATC3 is 1075 amino acids (aa) in length. It contains a calcineurin binding site (aa 109-114), two NLSs (aa 273-275 and 686‑688), one NES (aa 1032-1041) and an RHD that binds DNA (aa 433-592). There are multiple isoforms. Thirty amino acid, 33 aa and 10 aa substitutions for aa 1036‑1075 are known that may be accompanied by an alternate start site at Met480. Over aa 900-1035, human NFATC3 shares 93% aa identity with mouse NFATC3.

Long Name

Nuclear Factor of Activated T Cells C3

Alternate Names

NFAT4, NFATX

Entrez Gene IDs

4775 (Human); 18021 (Mouse)

Gene Symbol

NFATC3

UniProt

Q12968

Additional NFATC3 Products

Product Documents for Human NFATC3 Antibody (646115)

Certificate of Analysis

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Product Specific Notices for Human NFATC3 Antibody (646115)

For research use only

Citations for Human NFATC3 Antibody (646115)

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