Human NFATC3 Antibody

Catalog # Availability Size / Price Qty
MAB5834
MAB5834-SP
Human NFATC3 Antibody in Data
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Product Details
Citations (1)
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Human NFATC3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NFATC3 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 646115
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human NFATC3
Asp900-Asp1035
Accession # Q12968
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human NFATC3 antibody by Western Blot. View Larger

Detection of Human NFATC3 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Human NFATC3 Monoclonal Antibody (Catalog # MAB5834) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NFATC3 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFATC3

NFATC3 (Nuclear factor of activated T cells C3; also NFAT4 and NFATx) is a 145-150 kDa member of the NFAT family of transcription factors. NFATC3 is widely expressed being found in DP thymocytes, CD4+ T cells, smooth muscle cells, Schwann cells and mast cells. NFATC3 regulates gene transcription as part of a NFATC transcription complex. It is normally cytoplasmic and phosphorylated. Upon a rise in intracellular Ca+2, dephosphorylation occurs via calcineurin, and NFATC3 enters the nucleus. Human NFATC3 is 1075 amino acids (aa) in length. It contains a calcineurin binding site (aa 109-114), two NLSs (aa 273-275 and 686‑688), one NES (aa 1032-1041) and an RHD that binds DNA (aa 433-592). There are multiple isoforms. Thirty amino acid, 33 aa and 10 aa substitutions for aa 1036‑1075 are known that may be accompanied by an alternate start site at Met480. Over aa 900-1035, human NFATC3 shares 93% aa identity with mouse NFATC3.

Long Name
Nuclear Factor of Activated T Cells C3
Entrez Gene IDs
4775 (Human); 18021 (Mouse)
Alternate Names
NFAT4; NF-AT4; NFAT4nuclear factor of activated T-cells, cytoplasmic 3; NFATC3; NF-ATc3; NFATX; nuclear factor of activated T-cells c3 isoform IE-Xa; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3; T cell transcription factor NFAT4; T-cell transcription factor NFAT4

Product Datasheets

Citation for Human NFATC3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Novel Intergenically-spliced Chimera, NFATC3-PLA2G15, is Associated with Aggressive T-ALL Biology and Outcome
    Authors: J Bond, C Tran Quang, G Hypolite, M Belhocine, A Bergon, G Cordonnier, J Ghysdael, E Macintyre, N Boissel, S Spicuglia, V Asnafi
    Mol. Cancer Res., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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