Human NGFR/TNFRSF16 Antibody Summary
Accession # P08138
Human NGF R Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human/Mouse/Rat NGF R/TNFRSF16 ELISA Standard Curve. Recombinant Human/Mouse/Rat NGF R/TNFRSF16 (Catalog # 367-NR) was serially diluted 2-fold and captured by Goat Anti-Human/Mouse/Rat NGF R/TNFRSF16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF367) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse NGF R/TNFRSF16 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF367) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
p75 neurotrophin receptor, also named low affinity NGF receptor (NGF R), is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family. NGF R cDNA encodes a 427 amino acid (aa) residue precursor protein with a 28 aa residue signal peptide, a 222 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 155 aa residue intracellular domain. The extracellular region contains four cysteine-rich domains and binds NGF, BDNF, NT-3, and NT-4 approximately equally with low affinity. The cytoplasmic region contains a subtype 2 death domain.
NGF R expression has been shown to occur widely during development and in the adult. Expression has been detected in both neuronal and non-neuronal cells. NGF R was originally reported to function as a positive regulator of TrkA activity. NGF R has also been shown to signal by itself. Depending on its cellular environment, NGF R has now been shown to regulate cell migration, gene expression and to mediate apoptosis. Recombinant NGF R Fc chimera binds NGF with high affinity and is a potent NGF antagonist. Naturally occurring truncated NGF R containing the extracellular domain and lacking the transmembrane or intracellular domain has been detected in vivo in urine, plasma and in amniotic fluid of humans and rats.
- Barker, P.A. and R.A. Murphy (1992) Molecular and Cellular Biochemistry 110:1.
- Bamji, A.X. et al. (1998) J. Cell Biol. 140:911.
- Feinstein, E. et al. (1995) Trends Biochem. Sci. 20:342.
Citation for Human NGFR/TNFRSF16 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Direct and bystander killing of sarcomas by novel cytosine deaminase fusion gene.
Authors: Ramnaraine M, Pan W, Goblirsch M, Lynch C, Lewis V, Orchard P, Mantyh P, Clohisy DR
Cancer Res., 2003;63(20):6847-54.
Sample Types: Cell Lysates
Applications: Western Blot
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