Detection of Human and Mouse NGFRAP1/BEX3 by Western Blot.
Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NGFRAP1/BEX3 Monoclonal Antibody (Catalog # MAB6858) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NGFRAP1/BEX3 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
NGFRAP1/BEX3 in Human Prostate.
NGFRAP1/BEX3 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human NGFRAP1/BEX3 Monoclonal Antibody (Catalog # MAB6858) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
NGFRAP1 (nerve growth factor receptor associated protein 1), also called BEX3 (brain-expressed X-linked protein 3), NADE (p75NTR-associated death executor) or FAP-1 (Fas-associated phosphatase-1), is a 24 kDa member of the BEX protein family. A nuclear export signal (aa 77-87) allows NGFRAP1 to export bound p75NTR. It is widely expressed, but highest in ovarian granulosa cells, testis, prostate, seminal vesicle and liver. Except for a 13-19 amino acid (aa) rodent Arg/His region at aa 35 that is not found in humans, human NGFRAP1 shares 94% aa identity with mouse and rat NGFRAP1. In humans, a 101 aa isoform with an alternative start site at aa 11 has been described.
Nerve Growth Factor Receptor Associated Protein 1
Entrez Gene IDs:
27018 (Human); 12070 (Mouse); 117089 (Rat)
BEX3; BEX3p75NTR-associated cell death executor; Brain-expressed X-linked protein 3; DXS6984EX-linked 3; HGR74; NADE; nerve growth factor receptor (TNFRSF16) associated protein 1; Nerve growth factor receptor-associated protein 1; NGFRAP1; Ovarian granulosa cell 13.0 kDa protein HGR74; ovarian granulosa cell protein (13kD); protein BEX3
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