Human Nicotinamide N-Methyltransferase/NNMT Antibody

Catalog # Availability Size / Price Qty
MAB7736-100
MAB7736-SP
Human Nicotinamide N-Methyltransferase/NNMT Antibody in Western Blot
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Human Nicotinamide N-Methyltransferase/NNMT Antibody Summary

Species Reactivity
Human
Specificity
Detects human Nicotinamide N‑Methyltransferase/NNMT in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 954208
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E.coli-derived recombinant human Nicotinamide N‑Methyltransferase/NNMT
Met1-Leu264
Accession # P40261
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Nicotinamide N‑Methyltransferase/NNMT by Western Blot. View Larger

Detection of Nicotinamide N‑Methyltransferase/NNMT by Western Blot. Western blot shows lysates of human kidney tissue, human lung tissue, A549 human lung carcinoma cell line, and 786-O human renal cell adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Nicotinamide N-Methyltransferase/NNMT Monoclonal Antibody (Catalog # MAB7736) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Nicotinamide N-Methyltransferase/NNMT at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nicotinamide N-Methyltransferase/NNMT

Nicotinamide N-methyltransferase (NNMT) is a 264 aminoacids enzyme that catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. NNMT is found in human fat cells and the liver.  Significant up-regulation of NNMT was found pathologies like Parkinson's disease, cancer, atherosclerosis, and liver diseases.

Long Name
Nicotinamide N-Methyltransferase
Entrez Gene IDs
4837 (Human)
Alternate Names
EC 2.1.1.1; Nicotinamide NMethyltransferase; Nicotinamide N-Methyltransferase; NNMT

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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