Human Nicotinamide N-Methyltransferase/NNMT Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Nicotinamide N‑Methyltransferase/NNMT in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 954208
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E.coli-derived recombinant human Nicotinamide N‑Methyltransferase/NNMT
    Met1-Leu264
    Accession # P40261
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Nicotinamide N‑Methyltransferase/NNMT by Western Blot. Western blot shows lysates of human kidney tissue, human lung tissue, A549 human lung carcinoma cell line, and 786‑O human renal cell adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Nicotinamide N‑Methyltransferase/NNMT Monoclonal Antibody (Catalog # MAB7736) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Nicotinamide N‑Methyltransferase/NNMT at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nicotinamide N-Methyltransferase/NNMT
Nicotinamide N-methyltransferase (NNMT) is a 264 aminoacids enzyme that catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. NNMT is found in human fat cells and the liver.  Significant up-regulation of NNMT was found pathologies like Parkinson's disease, cancer, atherosclerosis, and liver diseases.
  • Long Name:
    Nicotinamide N-Methyltransferase
  • Entrez Gene IDs:
    4837 (Human)
  • Alternate Names:
    EC 2.1.1.1; Nicotinamide NMethyltransferase; nicotinamide N-methyltransferase; Nicotinamide N-Methyltransferase/NNMT; NNMT
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 22  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 5
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Molecular Weight Markers
Description Application Cat# Citations Images  

Full-Range Molecular Weight Marker, Biotinylated

MW001
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF007 16  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 8  
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Donkey Anti-Mouse IgG NL557 Affinity Purified PAb

Flow, IHC, ICC NL007 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 6
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 4
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Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF018 2  
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Mouse IgG Antibody

WB AF007 3
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 2  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 2
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 1
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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