Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 954208
View all Nicotinamide N-Methyltransferase/NNMT Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant human Nicotinamide N‑Methyltransferase/NNMT
Met1-Leu264
Accession # P40261

Specificity

Detects human Nicotinamide N‑Methyltransferase/NNMT in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Nicotinamide N‑Methyltransferase/NNMT Antibody

Detection of Nicotinamide N-Methyltransferase/NNMT antibody by Western Blot.

Detection of Nicotinamide N‑Methyltransferase/NNMT by Western Blot.

Western blot shows lysates of human kidney tissue, human lung tissue, A549 human lung carcinoma cell line, and 786-O human renal cell adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Nicotinamide N-Methyltransferase/NNMT Monoclonal Antibody (Catalog # MAB7736) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Nicotinamide N-Methyltransferase/NNMT at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Nicotinamide N‑Methyltransferase/NNMT Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human kidney tissue, human lung tissue, A549 human lung carcinoma cell line, and 786‑O human renal cell adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Nicotinamide N-Methyltransferase/NNMT

Nicotinamide N-methyltransferase (NNMT) is a 264 aminoacids enzyme that catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. NNMT is found in human fat cells and the liver.  Significant up-regulation of NNMT was found pathologies like Parkinson's disease, cancer, atherosclerosis, and liver diseases.

Long Name

Nicotinamide N-Methyltransferase

Alternate Names

Nicotinamide NMethyltransferase, NNMT

Entrez Gene IDs

4837 (Human)

Gene Symbol

NNMT

UniProt

P40261 (Human)

Additional Nicotinamide N-Methyltransferase/NNMT Products

Product Documents for Human Nicotinamide N‑Methyltransferase/NNMT Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Nicotinamide N‑Methyltransferase/NNMT Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Customer Reviews for Human Nicotinamide N‑Methyltransferase/NNMT Antibody

There are currently no reviews for this product. Be the first to review Human Nicotinamide N‑Methyltransferase/NNMT Antibody and earn rewards!

Have you used Human Nicotinamide N‑Methyltransferase/NNMT Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies