Human NKp30/NCR3 Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human NKp30.
  • Source
    Monoclonal Mouse IgG2A Clone # 210847
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse T cell hybridoma transfected with human NKp30 and Mouse myeloma cell line NS0-derived recombinant human NKp30 Fc Chimera
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Agonist Activity
    1-3 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Human NKp30 Antibody Induces IFN-gamma Secretion in
NK‑92 Cells.

Human NKp30 Monoclonal Antibody induces IFN-gamma secretion in the NK‑92 human natural killer lymphoma cell line in a dose-dependent manner, as measured using the Quantikine Human IFN-gamma ELISA Kit (Catalog # DIF50). The ED50 for this effect is typically
1‑3 μg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKp30/NCR3

NKp30, along with NKp44 and NKp46, constitute a group of receptors termed “Natural Cytotoxicity Receptors” (1). These receptors play a major role in triggering NK‑mediated killing of most tumor cells lines. NKp30 is a type I transmembrane protein having a single extracellular V-like immunoglobulin domain (2). A physical association with the ITAM-bearing accessory protein, CD3 zeta, occurs via a charged residue in the NKp30 transmembrane domain. Ligation of NKp30 with a specific antibody results in phosphorylation of CD3 zeta (3). NKp30 is expressed on both resting and activated NK cells of the CD56dim, CD16+ subset that account for more that 85% of NK cells found in peripheral blood and spleen (4). NKp30 is absent from the CD56bright, CD16- subset that constitutes the majority of NK cells in lymph node and tonsil, however, its expression is up-regulated in these cells upon IL-2 activation (4). Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK‑mediated lysis of immature dendritic cells (2, 5). The ligand(s) recognized by NKp30 has not been described.

  • References:
    1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
    2. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
    3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
    4. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
    5. Ferlazzo, G. et al. (2002) J. Exp. Med. 195:343.
  • Entrez Gene IDs:
    259197 (Human); 294251 (Rat)
  • Alternate Names:
    Activating natural killer receptor p30; CD337 antigen; CD337; CD337activating NK-A1 receptor; LY117,1C7NK-p30; lymphocyte antigen 117; natural cytotoxicity triggering receptor 3; Natural killer cell p30-related protein; NCR3; NKp30; NKp30MALS
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure.
    Authors: Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D
    PLoS ONE, 2015;10(8):e0134568.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  2. Natural killer cells are essential for the ability of BRAF inhibitors to control BRAFV600E-mutant metastatic melanoma.
    Authors: Ferrari de Andrade L, Ngiow S, Stannard K, Rusakiewicz S, Kalimutho M, Khanna K, Tey S, Takeda K, Zitvogel L, Martinet L, Smyth M
    Cancer Res, 2014;74(24):7298-308.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  3. NKp44 receptor mediates interaction of the envelope glycoproteins from the West Nile and dengue viruses with NK cells.
    Authors: Hershkovitz O, Rosental B, Rosenberg LA, Navarro-Sanchez ME, Jivov S, Zilka A, Gershoni-Yahalom O, Brient-Litzler E, Bedouelle H, Ho JW, Campbell KS, Rager-Zisman B, Despres P, Porgador A
    J. Immunol., 2009;183(4):2610-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  4. Many NK cell receptors activate ERK2 and JNK1 to trigger microtubule organizing center and granule polarization and cytotoxicity.
    Authors: Chen X, Trivedi PP, Ge B, Krzewski K, Strominger JL
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(15):6329-34.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  5. A novel binding assay to assess specificity of monoclonal antibodies.
    Authors: Warren HS, Warren&lt;/LastName&gt;&lt;ForeNam HS&lt;/Initia, Bettadapura J
    J. Immunol. Methods, 2005;305(1):33-8.
    Species: Human
    Sample Type: Complex Sample Type
    Application: Epitope Mapping
Isotype Controls
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Mouse IgG2A Isotype Control

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Mouse IgG2A Isotype Control

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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Mouse IgG Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Mouse IgG2A Antibody

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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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