Human NKp30/NCR3 Antibody

Catalog # Availability Size / Price Qty
Human NKp30 Antibody Induces IFN-gamma  Secretion in NK‑92 Cells.
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Product Details
Citations (8)
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Reviews (1)

Human NKp30/NCR3 Antibody Summary

Species Reactivity
Detects human NKp30.
Monoclonal Mouse IgG2A Clone # 210847
Protein A or G purified from hybridoma culture supernatant
Mouse T cell hybridoma transfected with human NKp30 and Mouse myeloma cell line NS0-derived recombinant human NKp30 Fc Chimera
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

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Recommended Concentration
Agonist Activity
1-3 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Agonist Activity Human NKp30 Antibody Induces IFN-? Secretion antibody in NK-92 Cells. View Larger

Human NKp30 Antibody Induces IFN-gamma Secretion in NK‑92 Cells. Human NKp30 Monoclonal Antibody induces IFN-gamma secretion in the NK-92 human natural killer lymphoma cell line in a dose-dependent manner, as measured using the Human IFN-gamma DuoSet ELISA Kit (Catalog # DY285B). The ED50 for this effect is typically 1-3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKp30/NCR3

NKp30, along with NKp44 and NKp46, constitute a group of receptors termed “Natural Cytotoxicity Receptors” (1). These receptors play a major role in triggering NK‑mediated killing of most tumor cells lines. NKp30 is a type I transmembrane protein having a single extracellular V-like immunoglobulin domain (2). A physical association with the ITAM-bearing accessory protein, CD3 zeta, occurs via a charged residue in the NKp30 transmembrane domain. Ligation of NKp30 with a specific antibody results in phosphorylation of CD3 zeta (3). NKp30 is expressed on both resting and activated NK cells of the CD56dim, CD16+ subset that account for more that 85% of NK cells found in peripheral blood and spleen (4). NKp30 is absent from the CD56bright, CD16- subset that constitutes the majority of NK cells in lymph node and tonsil, however, its expression is up-regulated in these cells upon IL-2 activation (4). Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK‑mediated lysis of immature dendritic cells (2, 5). The ligand(s) recognized by NKp30 has not been described.

  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
  2. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
  4. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  5. Ferlazzo, G. et al. (2002) J. Exp. Med. 195:343.
Entrez Gene IDs
259197 (Human); 294251 (Rat); 102133932 (Cynomolgus Monkey)
Alternate Names
Activating natural killer receptor p30; CD337 antigen; CD337; CD337activating NK-A1 receptor; LY117,1C7NK-p30; lymphocyte antigen 117; natural cytotoxicity triggering receptor 3; Natural killer cell p30-related protein; NCR3; NKp30; NKp30MALS

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Citations for Human NKp30/NCR3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Morphological profiling of human T and NK lymphocytes by high-content cell imaging
    Authors: Y German, L Vulliard, A Kamnev, L Pfajfer, J Huemer, AK Mautner, A Rubio, A Kalinichen, K Boztug, A Ferrand, J Menche, L Dupré
    Cell Reports, 2021;36(1):109318.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Nanoscale Dynamism of Actin Enables Secretory Function in Cytolytic Cells
    Authors: AF Carisey, EM Mace, MB Saeed, DM Davis, JS Orange
    Curr. Biol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. NKp30 isoforms and NKp46 transcripts in metastatic melanoma patients: Unique NKp30 pattern in rare melanoma patients with favorable evolution
    Authors: M Messaouden, G Fregni, D Enot, N Jacquelot, E Neves, N Germaud, HJ Garchon, W Boukouaci, R Tamouza, J Chanal, MF Avril, A Toubert, L Zitvogel, S Rusakiewic, A Caignard
    Oncoimmunology, 2016;5(12):e1154251.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  4. NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure.
    Authors: Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D
    PLoS ONE, 2015;10(8):e0134568.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  5. Natural killer cells are essential for the ability of BRAF inhibitors to control BRAFV600E-mutant metastatic melanoma.
    Authors: Ferrari de Andrade L, Ngiow S, Stannard K, Rusakiewicz S, Kalimutho M, Khanna K, Tey S, Takeda K, Zitvogel L, Martinet L, Smyth M
    Cancer Res, 2014;74(24):7298-308.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  6. NKp44 receptor mediates interaction of the envelope glycoproteins from the West Nile and dengue viruses with NK cells.
    Authors: Hershkovitz O, Rosental B, Rosenberg LA, Navarro-Sanchez ME, Jivov S, Zilka A, Gershoni-Yahalom O, Brient-Litzler E, Bedouelle H, Ho JW, Campbell KS, Rager-Zisman B, Despres P, Porgador A
    J. Immunol., 2009;183(4):2610-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  7. Many NK cell receptors activate ERK2 and JNK1 to trigger microtubule organizing center and granule polarization and cytotoxicity.
    Authors: Chen X, Trivedi PP, Ge B, Krzewski K, Strominger JL
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(15):6329-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  8. A novel binding assay to assess specificity of monoclonal antibodies.
    Authors: Warren HS, Warren&lt;/LastName&gt;&lt;ForeNam HS&lt;/Initia, Bettadapura J
    J. Immunol. Methods, 2005;305(1):33-8.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: Epitope Mapping


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Human NKp30/NCR3 Antibody
By Anonymous on 12/11/2020
Application: Func Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

Good antibody worked as mentioned in data sheet. Leads to activation of NK cells.