Human NKp30/NCR3 Antibody (210847)

R&D Systems | Catalog # MAB18491

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Agonist Activity

Cited:

Western Blot, Bioassay, Epitope Mapping, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 210847
View all NKp30/NCR3 Primary Antibodies »

Product Specifications

Immunogen

Mouse T cell hybridoma transfected with human NKp30 and Mouse myeloma cell line NS0-derived recombinant human NKp30 Fc Chimera

Specificity

Detects human NKp30.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human NKp30/NCR3 Antibody (210847)

Human NKp30 Antibody Induces IFN-? Secretion antibody in NK-92 Cells.

Human NKp30 Antibody Induces IFN-gamma Secretion in NK‑92 Cells.

Human NKp30 Monoclonal Antibody induces IFN-gamma secretion in the NK-92 human natural killer lymphoma cell line in a dose-dependent manner, as measured using the Human IFN-gamma DuoSet ELISA Kit (Catalog # DY285B). The ED50 for this effect is typically 1-3 µg/mL.

Applications for Human NKp30/NCR3 Antibody (210847)

Application
Recommended Usage

Agonist Activity

1-3 µg/mL
Sample: NK-92 human natural killer lymphoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB18491 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NKp30/NCR3

NKp30, along with NKp44 and NKp46, constitute a group of receptors termed “Natural Cytotoxicity Receptors” (1). These receptors play a major role in triggering NK‑mediated killing of most tumor cells lines. NKp30 is a type I transmembrane protein having a single extracellular V-like immunoglobulin domain (2). A physical association with the ITAM-bearing accessory protein, CD3 zeta, occurs via a charged residue in the NKp30 transmembrane domain. Ligation of NKp30 with a specific antibody results in phosphorylation of CD3 zeta (3). NKp30 is expressed on both resting and activated NK cells of the CD56dim, CD16+ subset that account for more that 85% of NK cells found in peripheral blood and spleen (4). NKp30 is absent from the CD56bright, CD16- subset that constitutes the majority of NK cells in lymph node and tonsil, however, its expression is up-regulated in these cells upon IL-2 activation (4). Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK‑mediated lysis of immature dendritic cells (2, 5). The ligand(s) recognized by NKp30 has not been described.

References

  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
  2. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
  4. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  5. Ferlazzo, G. et al. (2002) J. Exp. Med. 195:343.

Alternate Names

CD337, NCR3

Entrez Gene IDs

259197 (Human); 294251 (Rat); 102133932 (Cynomolgus Monkey)

Gene Symbol

NCR3

Additional NKp30/NCR3 Products

Product Documents for Human NKp30/NCR3 Antibody (210847)

Certificate of Analysis

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Product Specific Notices for Human NKp30/NCR3 Antibody (210847)

For research use only

Citations for Human NKp30/NCR3 Antibody (210847)

Customer Reviews for Human NKp30/NCR3 Antibody (210847) (1)

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  • Human NKp30/NCR3 Antibody
    Name: Anonymous
    Application: Functional Assay
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 12/11/2020
    Good antibody worked as mentioned in data sheet. Leads to activation of NK cells.
    Human NKp30/NCR3 Antibody (210847) MAB18491

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