Human NKX2.5 Antibody
Human NKX2.5 Antibody Summary
Gln24-Arg137
Accession # P52952
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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NKX2.5 in Human Heart. NKX2.5 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human NKX2.5 Monoclonal Antibody (Catalog # MAB2444) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKX2.5
NKX2.5, also called NKX-2E or Csx (Cardiac specific homeobox protein), is a transcriptional activator. Its expression is essential for normal cardiovascular development. During embryonic development, NKX2.5 is also expressed in the foregut, thyroid, spleen, and stomach, while in the adult expression is predominantly restricted to the heart.
Product Datasheets
Citations for Human NKX2.5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State
Authors: L Zhang, J Avery, A Yin, AM Singh, TS Cliff, H Yin, S Dalton
Cell Stem Cell, 2020;0(0):.
Species: Human
Sample Types: Organoid
Applications: IHC -
Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State
Authors: L Zhang, J Avery, A Yin, AM Singh, TS Cliff, H Yin, S Dalton
Cell Stem Cell, 2020;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The involvement of protein kinase C-? in isoflurane induced preconditioning of human embryonic stem cell--derived Nkx2.5(+) cardiac progenitor cells
Authors: IA Song, AY Oh, JH Kim, YM Choi, YT Jeon, JH Ryu, JW Hwang
BMC Anesthesiol, 2016;16(0):13.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Essential and unexpected role of Yin Yang 1 to promote mesodermal cardiac differentiation.
Authors: Gregoire S, Karra R, Passer D, Deutsch M, Krane M, Feistritzer R, Sturzu A, Domian I, Saga Y, Wu S
Circ Res, 2013;112(6):900-10.
Species: Mouse
Sample Types: Embryo
Applications: IHC -
Dedifferentiated fat cells convert to cardiomyocyte phenotype and repair infarcted cardiac tissue in rats.
Authors: Jumabay M, Matsumoto T, Yokoyama S, Kano K, Kusumi Y, Masuko T, Mitsumata M, Saito S, Hirayama A, Mugishima H, Fukuda N
J. Mol. Cell. Cardiol., 2009;47(5):565-75.
Species: Rat
Sample Types: Whole Cells
Applications: ICC
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Average Rating: 4 (Based on 1 Review)
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