Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
78.1-5000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human Notch-1 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)
Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)
Microplates: (Catalog # DY990), or equivalent
Plate Sealers: (Catalog # DY992), or equivalent
*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.
Background: Notch-1
Notch proteins (so named for 'notches' in fly wings) are type I transmembrane glycoproteins involved in specifying cell fates and defining boundaries between different cell types during developmental processes. Notch extracellular domains are large, having 34-36 EGF-like repeats followed by three notch/Lin-12 repeats. Notch proteins interact with transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand binding, a series of cleavage events results in the release of the Notch intracellular domain (NICD), which translocates to the nucleus and initiates transcription of Notch-responsive genes. Thus, Notch acts as both a ligand-binding receptor and a nuclear factor that regulates transcription.
The four mammalian Notch receptors appear to have distinct functions, since they do not compensate for one another in genetic studies. Mutations in Notch receptors also lead to specific developmental disorders. For example, Notch-3 is predominantly expressed in the developing central nervous system of mice. Mutations in Notch-3 in humans cause an autosomal dominant condition called CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy). This disorder is characterized by recurrent ischemic strokes at an early age without any underlying vascular risk and progressive dementia.
Alternate Names
Notch1, TAN1
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