Human NQO-1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human NQO-1 by Western Blot.
1 Image
Product Details
Supplemental Products

Human NQO-1 Antibody Summary

Species Reactivity
Detects human NQO-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human NQO-2 is observed.
Monoclonal Mouse IgG2B Clone # 844142
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human NQO-1
Accession # P15559
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below
25 µg/mL
Cell lysates spiked with recombinant human NQO-1, see our available Western blot detection antibodies.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human NQO-1 antibody by Western Blot. View Larger

Detection of Human NQO-1 by Western Blot. Western blot shows lysates of human lung tissue, human kidney tissue, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human NQO-1 Monoclonal Antibody (Catalog # MAB7567) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NQO-1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NQO-1

NQO-1 (NADPH Quinone acceptor Oxidoreductase 1; also DTD and Menadione reductase) is a 30-33 kDa cytoplasmic member of the NAD(P)H dehydrogenase family of enzymes. It is widely expressed, being found in adipocytes, endothelial cells and hepatocytes. NQO-1 serves as a cytoprotective molecule, reducing quinones (atmospheric benzene and esrtrogen compounds) and nitroaromatics without consuming sulfhydryl compounds. It also acts as a backup superoxide scavenger to SOD. NQO-1 reportedly binds and stabilizes key cell protection molecules such as p53, thus acting as a gatekeeper for proteosome-mediated protein turnover. Human NQO-1 is 274 amino acids (aa) in length. It contains an FMN reductase domain (aa 5-212) and two utilized Lys acetylation sites. NQO-1 functions as a noncovalent homodimer. There are three potential at Met22, while two others show a deletion of aa 141-174 and 102-139, respectively. Full length human NQO-1 shares 86% aa sequence identity with mouse NQO-1.

Long Name
NAD(P)H Dehydrogenase, Quinone 1
Entrez Gene IDs
1728 (Human)
Alternate Names
Azoreductase; DHQU; DIA4; DIA4azoreductase; diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase-4; Dioxin-inducible 1; DTD; DTDEC; DT-diaphorase; Menadione Reductase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase, quinone 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NMOR1; NMOR1diaphorase-4; NQO1; NQO-1; Phylloquinone reductase; QR1; QR1NMORI; Quinone Reductase 1

Product Datasheets


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human NQO-1 Antibody

There are currently no reviews for this product. Be the first to review Human NQO-1 Antibody and earn rewards!

Have you used Human NQO-1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review