|Detection of Human NTAL by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and THP‑1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human NTAL Monoclonal Antibody (Catalog # MAB4066) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NTAL at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Intracellular Staining by Flow Cytometry
|Detection of NTAL in THP-1 Human Cell Line by Flow Cytometry. THP‑1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human NTAL Monoclonal Antibody (Catalog # MAB4066, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.|
Non-T cell activation linker (NTAL), also known as linker for activation of B cells (LAB), is a transmembrane adaptor protein involved in immunoreceptor signaling. NTAL is expressed in lipid raft microdomains of B cells, mast cells, monocytes and NK cells. Rapid tyrosine phosphorylation of NTAL occurs upon BCR aggregation in B cells, Fc epsilon RI aggregation and Kit activation in mast cells, and Fc gamma RI aggregation in monocytes. Phosphorylated NTAL recruits signaling molecules such as Grb2, Gab1, and c-Cbl into receptor-signaling complexes. Defects in the NTAL gene may cause Williams-Beuren syndrome, a rare genetic disorder characterized by mild mental retardation, and abnormalities in the cardiovascular and musculo-skeletal systems.
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